Kuzin B, Roberts I, Peunova N, Enikolopov G
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Cell. 1996 Nov 15;87(4):639-49. doi: 10.1016/s0092-8674(00)81384-7.
Cell division and subsequent programmed cell death in imaginal discs of Drosophila larvae determine the final size of organs and structures of the adult fly. We show here that nitric oxide (NO) is involved in controlling the size of body structures during Drosophila development. We have found that NO synthase (NOS) is expressed at high levels in developing imaginal discs. Inhibition of NOS in larvae causes hypertrophy of organs and their segments in adult flies, whereas ectopic expression of NOS in larvae has the opposite effect. Blocking apoptosis in eye imaginal discs unmasks surplus cell proliferation and results in an increase in the number of ommatidia and component cells of individual ommatidia. These results argue that NO acts as an antiproliferative agent during Drosophila development, controlling the balance between cell proliferation and cell differentiation.
果蝇幼虫成虫盘的细胞分裂及随后的程序性细胞死亡决定了成年果蝇器官和结构的最终大小。我们在此表明，一氧化氮（NO）参与果蝇发育过程中身体结构大小的控制。我们发现，一氧化氮合酶（NOS）在发育中的成虫盘中高水平表达。抑制幼虫中的NOS会导致成年果蝇的器官及其节段肥大，而在幼虫中异位表达NOS则会产生相反的效果。阻断眼成虫盘中的细胞凋亡会揭示出多余的细胞增殖，并导致小眼数量以及单个小眼的组成细胞数量增加。这些结果表明，NO在果蝇发育过程中作为一种抗增殖剂，控制着细胞增殖与细胞分化之间的平衡。
