Mombaerts P, Wang F, Dulac C, Chao S K, Nemes A, Mendelsohn M, Edmondson J, Axel R
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute and Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA.
Cell. 1996 Nov 15;87(4):675-86. doi: 10.1016/s0092-8674(00)81387-2.
We have developed a genetic approach to visualize axons from olfactory sensory neurons expressing a given odorant receptor, as they project to the olfactory bulb. Neurons expressing a specific receptor project to only two topographically fixed loci among the 1800 glomeruli in the mouse olfactory bulb. Our data provide direct support for a model in which a topographic map of receptor activation encodes odor quality in the olfactory bulb. Receptor swap experiments suggest that the olfactory receptor plays an instructive role in the guidance process but cannot be the sole determinant in the establishment of this map. This genetic approach may be more broadly applied to visualize the development and plasticity of projections in the mammalian nervous system.
我们已经开发出一种遗传学方法，用于观察表达特定气味受体的嗅觉感觉神经元的轴突向嗅球投射的过程。表达特定受体的神经元仅投射到小鼠嗅球1800个肾小球中两个拓扑固定的位点。我们的数据为一种模型提供了直接支持，该模型认为受体激活的拓扑图在嗅球中编码气味质量。受体交换实验表明，嗅觉受体在引导过程中起指导作用，但不是建立该图谱的唯一决定因素。这种遗传学方法可能更广泛地应用于观察哺乳动物神经系统中投射的发育和可塑性。
