一项关于电泳、免疫电泳、免疫固定电泳和比浊法在血清蛋白检测中相对效用的回顾性研究。

A retrospective study of the relative utility of electrophoresis, immunoelectrophoresis, immunofixation, and nephelometry in the investigation of serum proteins.

作者信息

Salkie M L

机构信息

Department of Laboratory Medicine and Pathology, University of Alberta Hospitals, Edmonton, Canada.

出版信息

Clin Biochem. 1996 Feb;29(1):39-42. doi: 10.1016/0009-9120(95)02002-0.

DOI:10.1016/0009-9120(95)02002-0

PMID:8929822

Abstract

OBJECTIVES

To assess the relative value of visual inspection of protein electrophoresis patterns, immunoelectrophoresis, immunofixation, and specific protein quantitation in the investigation of serum proteins.

DESIGN AND METHODS

A retrospective study of protein results on 372 sera analyzed by electrophoresis and immunoelectrophoresis. A subsection of specimens (276) also had immunoglobulin quantitation by immunonephelometry and 30 were studied by immunofixation.

RESULTS

Total gamma globulin levels were correctly assessed visually in 84.4% of cases. Discrete bands seen visually were mainly monoclonal in 74 of 80 (92.5%). Six monoclonal proteins were not detected visually. Twenty-five monoclonal proteins required immunofixation for typing, and 60 were typed by immunoelectrophoresis. The visual assessment of the level of normal immunoglobulins in the presence of a monoclonal gammopathy was correct in 58% of cases.

CONCLUSIONS

The majority of monoclonal gammopathies could be evaluated by a combination of protein electrophoresis and immunoelectrophoresis. Except for quantitating monoclonal immunoglobins, protein electrophoresis was unreliable as a quantitative procedure and nephelometry was preferred.

摘要

目的

评估蛋白质电泳图谱的目视检查、免疫电泳、免疫固定电泳及特定蛋白质定量在血清蛋白研究中的相对价值。

设计与方法

对372份血清进行电泳和免疫电泳分析，并对蛋白质结果进行回顾性研究。部分标本（276份）还采用免疫比浊法进行免疫球蛋白定量分析，30份标本采用免疫固定电泳进行研究。

结果

84.4%的病例中，总γ球蛋白水平通过目视检查得到正确评估。目视可见的离散条带在80条中有74条（92.5%）主要为单克隆条带。6条单克隆蛋白未通过目视检查检测到。25条单克隆蛋白需要通过免疫固定电泳进行分型，60条通过免疫电泳进行分型。在存在单克隆丙种球蛋白病的情况下，58%的病例中正常免疫球蛋白水平的目视评估是正确的。