Coughlin D J, Valdes L, Rome L C
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
J Exp Biol. 1996 Feb;199(Pt 2):459-63. doi: 10.1242/jeb.199.2.459.
Recent attempts to determine how fish muscles are used to power swimming have employed the work loop technique (driving isolated muscles using their in vivo strain and stimulation pattern). These muscle strains have in turn been determined from the anatomical high-speed cine technique. In this study, we used an independent technique, sonomicrometry, to attempt to verify these strain measurements and the conclusions based on them. We found that the strain records measured from sonomicrometry and the anatomical-cine techniques were very similar. The ratio of the strain measured from sonomicrometry to that from the anatomical-cine technique was remarkably close to unity (1.046 +/- 0.013, mean +/- S.E.M., N = 15, for transducers placed on the muscle surface and corrected for muscle depth, and 0.921 +/- 0.028, N = 8, in cases where the transducers were inserted to the average depth of the red muscle). These measurements also showed that red muscle shortening occurs simultaneously with local backbone curvature, unlike previous results which suggested that white muscle shortening during the escape response occurs prior to the change in local backbone curvature.
最近，为了确定鱼类肌肉如何为游泳提供动力，人们采用了工作循环技术（利用其体内应变和刺激模式驱动分离的肌肉）。这些肌肉应变反过来又通过解剖学高速电影技术来确定。在本研究中，我们使用了一种独立的技术——超声微测技术，试图验证这些应变测量结果以及基于这些结果得出的结论。我们发现，通过超声微测技术和解剖学电影技术测量得到的应变记录非常相似。超声微测技术测量的应变与解剖学电影技术测量的应变之比非常接近1（对于放置在肌肉表面并校正肌肉深度的换能器，为1.046±0.013，平均值±标准误，N = 15；对于换能器插入到红肌平均深度的情况，为0.921±0.028，N = 8）。这些测量结果还表明，红肌缩短与局部脊柱弯曲同时发生，这与之前的结果不同，之前的结果表明逃避反应期间白肌缩短发生在局部脊柱弯曲变化之前。
