Ogaard B, Duschner H, Ruben J, Arends J
Department of Orthodontics, University of Oslo, Norway.
Eur J Oral Sci. 1996 Aug;104(4 ( Pt 1)):378-83. doi: 10.1111/j.1600-0722.1996.tb00095.x.
The aim of the present investigation was to combine 2 techniques suitable for lesion characterization: quantitative microradiography (TMR) and confocal laser scanning microscopy (CLSM) on in vivo induced lesions with and without a fluoride varnish (Duraphat) treatment. Orthodontic bands were attached to premolars to be extracted for orthodontic reasons to induce enamel caries on the buccal surfaces. In the caries development part of the study, 1 tooth of each pair received at the start 1 topical application with the fluoride varnish Duraphat, the contralateral tooth serving as untreated control. All teeth were extracted after 4 weeks. In the caries progression part, the premolars were banded for 4 weeks without any treatment to induce caries, 1 premolar in each pair was then extracted and analyzed. The contralateral tooth received one Duraphat application and was extracted after another 2 weeks. In the caries development part of the study, TMR analysis showed that the Duraphat treatment on sound enamel reduced lesion depth by 48% compared to the untreated control. In the caries progression part, no significant difference was found between the untreated teeth extracted after 4 weeks and the fluoride treated lesions extracted after another 2 weeks. CLSM images agreed well with the TMR measurements. In the fluoride treated lesions, the CLSM images showed more light scattering indicating precipitation of material.
定量显微放射照相术（TMR）和共聚焦激光扫描显微镜（CLSM），用于对体内诱导产生的、有或没有使用含氟涂料（Duraphat）处理的病变进行研究。正畸带环被粘接到因正畸原因需要拔除的前磨牙上，以在颊面诱导釉质龋。在该研究的龋病发展部分，每对牙齿中的一颗在开始时接受一次Duraphat含氟涂料的局部应用，对侧牙齿作为未处理的对照。4周后所有牙齿均被拔除。在龋病进展部分，前磨牙被带环4周不进行任何处理以诱导龋病，然后每对中的一颗前磨牙被拔除并进行分析。对侧牙齿接受一次Duraphat应用，并在另外2周后被拔除。在该研究的龋病发展部分，TMR分析表明，与未处理的对照相比，在健康釉质上使用Duraphat处理可使病变深度降低48%。在龋病进展部分，4周后拔除的未处理牙齿与另外2周后拔除的经氟处理的病变之间未发现显著差异。CLSM图像与TMR测量结果吻合良好。在经氟处理的病变中，CLSM图像显示出更多的光散射，表明有物质沉淀。
