Lescroart O, Roelants I, Mikolajczyk T, Bosma P T, Schulz R W, Kühn E R, Ollevier F
Laboratory of Comparative Endocrinology, Catholic University of Leuven, Louvain, Belgium.
Gen Comp Endocrinol. 1996 Nov;104(2):147-55. doi: 10.1006/gcen.1996.0157.
A highly sensitive radioimmunoassay has been developed for measuring plasma growth hormone (GH) concentrations in the African catfish (Clarias gariepinus). The lower detection limit of the assay was 0.1 ng/ml and the standard curve had an ED50 value of 0.5 ng/ml. The validity of the assay was established and the effects of several neurotransmitters on the release of GH were examined. In vitro experiments, using a static culture system for dispersed pituitary cells, demonstrated that the GH release in African catfish was affected by growth hormone-releasing hormone and somatostatin. Single intraperitoneal injections with a dopamine agonist, apomorphine, produced significant and dose-dependent increases in plasma GH levels. Unlike carp, goldfish, and tilapia, a super-active analogue of salmon gonadotrophin-releasing hormone did not alter plasma GH levels in African catfish.
已开发出一种高灵敏度放射免疫分析法，用于测定非洲鲶鱼（Clarias gariepinus）血浆中生长激素（GH）的浓度。该分析方法的检测下限为0.1 ng/ml，标准曲线的ED50值为0.5 ng/ml。确定了该分析方法的有效性，并研究了几种神经递质对GH释放的影响。使用分散垂体细胞的静态培养系统进行的体外实验表明，非洲鲶鱼体内的GH释放受生长激素释放激素和生长抑素的影响。单次腹腔注射多巴胺激动剂阿扑吗啡可使血浆GH水平显著且呈剂量依赖性升高。与鲤鱼、金鱼和罗非鱼不同，鲑鱼促性腺激素释放激素的超活性类似物不会改变非洲鲶鱼的血浆GH水平。
