Bernstein J J, Karp S M, Goldberg W J, DeGiorgio L A, Blass J P
Laboratory of Central Nervous System Injury and Regeneration, Department of Veterans Affairs Medical Center, Washington, DC 20422, USA.
Int J Dev Neurosci. 1996 Aug;14(5):681-7. doi: 10.1016/0736-5748(95)00096-8.
The following series of experiments explores the post-xenografting differentiation of a naturally occurring, non-neuronal cell cultured from the leptomeninges of an 84-year-old woman. In culture, flat process-bearing human cells from the leptomeninges were positive for GFAP and 200 kDa neurofilament protein (negative for 68, 160 kDa neurofilament protein). The C3 spinal cord was exposed in 30 adult athymic rats. The hindlimb dorsal columns were transected at C3 and the nerve fibers aspirated to form a pocket, into which 10(6) fast blue-labeled, human leptomeningeal-derived cells were placed. The C3 spinal cord was studied immunohistochemically over 60 days. Three days later the dorsal horn contained fast blue-GFAP-positive astrocyte-like cells that were negative for neurofilament protein. By 7 days, large, process-bearing, fast blue-GFAP-positive (neurofilament protein-negative), astrocyte-like cells joined the native astrocytes of the pia-glia membrane and were in the gray matter of the spinal cord. Some of these astrocyte-like cells were also positive for the human specific histocompatibility complex, HLADR. These data extend the age, species and tissue of origin for pluripotential cells for CNS transplantation.
以下一系列实验探究了从一名84岁女性软脑膜培养的天然非神经元细胞在异种移植后的分化情况。在培养过程中，来自软脑膜的带有扁平突起的人类细胞GFAP和200 kDa神经丝蛋白呈阳性（68 kDa、160 kDa神经丝蛋白呈阴性）。对30只成年无胸腺大鼠暴露其C3脊髓。在C3水平横断后肢背柱并吸出神经纤维以形成一个腔隙，向其中植入10⁶个经快蓝标记的源自人类软脑膜的细胞。在60天内对C3脊髓进行免疫组织化学研究。三天后，背角含有快蓝-GFAP阳性的星形胶质细胞样细胞，神经丝蛋白呈阴性。到第7天，大型的、带有突起的、快蓝-GFAP阳性（神经丝蛋白阴性）的星形胶质细胞样细胞与软膜-胶质膜的天然星形胶质细胞汇合，并位于脊髓灰质中。其中一些星形胶质细胞样细胞人类特异性组织相容性复合体HLADR也呈阳性。这些数据扩展了用于中枢神经系统移植的多能细胞的年龄、物种和组织来源范围。
