DeGiorgio L A, Bernstein J J, Blass J P
Altshul Laboratory for Dementia Research, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605, USA.
Int J Dev Neurosci. 1997 Apr;15(2):231-8. doi: 10.1016/s0736-5748(96)00076-7.
Since previous studies have shown that cells cultured from human leptomeninges can express neuronal and glial antigens under appropriate culture conditions [DeGiorgio L. A. et al. (1994) J. Neurol. Sci. 124, 141 148; Bernstein J. J. et al. (1996) Int. J. Derl Neurosci. 14(5), 681 687], we have studied the developmental characteristics of these cells further by grafting them into young adult rat brains. Cells were labeled in culture with Fast Blue and were identified unequivocally by hybridization with nick-translated human DNA. Intensely Fast Blue positive human leptomeningeal cells were concentrated in the implant pocket and adjacent rat leptomeninges al one and two weeks postimplant. Human and rat leptomeningeal cells were similar morphologically and were equally immunopositive for vimentin and fibronectin. Implanted human cells did not express the neuronal and glial proteins they had in vitro. Cells which hybridized with human DNA corresponded to the intensely Fast Blue positive cells. Small groups of human DNA hybridizing cells were also observed in the choroid plexus. Less intensely Fast Blue positive neurons and glia were found in the brain but these hybridized with rat DNA. A minority of human leptomeningeal cells implanted into rat brain are subsequently found in host leptomeninges where they demonstrate properties characteristic of leptomeningeal fibroblasts. Small numbers of implanted cells can survive for two weeks.
由于先前的研究表明，在适当的培养条件下，从人软脑膜培养的细胞可以表达神经元和神经胶质抗原[DeGiorgio L. A.等人（1994年）《神经科学杂志》124卷，第141 - 148页；Bernstein J. J.等人（1996年）《国际发育神经科学杂志》14(5)卷，第681 - 687页]，我们通过将这些细胞移植到成年幼鼠大脑中，进一步研究了它们的发育特征。细胞在培养中用快蓝标记，并通过与切口平移的人DNA杂交进行明确鉴定。在植入后1周和2周时，强烈快蓝阳性的人软脑膜细胞集中在植入腔和相邻的大鼠软脑膜中。人和大鼠的软脑膜细胞在形态上相似，对波形蛋白和纤连蛋白的免疫阳性程度相同。植入的人细胞在体外未表达神经元和神经胶质蛋白。与人类DNA杂交的细胞对应于强烈快蓝阳性细胞。在脉络丛中也观察到少量与人类DNA杂交的细胞。在大脑中发现了少量快蓝阳性程度较低的神经元和神经胶质，但它们与大鼠DNA杂交。植入大鼠大脑的少数人软脑膜细胞随后在宿主软脑膜中被发现，在那里它们表现出软脑膜成纤维细胞的特征特性。少量植入细胞可以存活两周。
