Weindlmayr-Goettel M, Weberhofer G, Gilly H, Kress H G
Department B of Anaesthesiology and General Intensive Care Medicine, University of Vienna, Allgemeines Krankenhaus, Austria.
J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):123-7. doi: 10.1016/0378-4347(96)00157-0.
An assay has been developed and validated for the routine monitoring of mivacurium in plasma. It consists of liquid-liquid extraction with dichloromethane and high-performance liquid chromatography with fluorometric detection (excitation and emission wavelengths 220 nm and 320 nm, respectively). A Spherisorb C, 5 microns column and a mobile phase containing acetonitrile, KH2PO4 and methanol are used. At a flow-rate of 1 ml/min, a concentration gradient is applied. The detection limit is approximately 1 ng/ml in plasma. For the separation of steroisomeres, the Spherisorb SCX 10 microns column and acetonitrile-Na2SO4 as a mobile phase can be used. The assay shows good linearity over the range 1-1000 ng/ml. The accuracy and precision allows the utilisation in clinical pharmacokinetic studies.
已开发并验证了一种用于常规监测血浆中米库氯铵的分析方法。该方法包括用二氯甲烷进行液液萃取以及采用荧光检测的高效液相色谱法（激发波长和发射波长分别为220nm和320nm）。使用一根5微米的Spherisorb C柱以及含有乙腈、磷酸二氢钾和甲醇的流动相。在流速为1ml/分钟时，施加浓度梯度。血浆中的检测限约为1ng/ml。为分离立体异构体，可使用10微米的Spherisorb SCX柱以及乙腈 - 硫酸钠作为流动相。该分析方法在1 - 1000ng/ml范围内显示出良好的线性。其准确性和精密度使其可用于临床药代动力学研究。
