Gao H, Roy S, Donati F, Varin F
Faculté de Pharmacie, Université de Montréal, Quebec, Canada.
J Chromatogr B Biomed Sci Appl. 1998 Oct 23;718(1):129-34. doi: 10.1016/s0378-4347(98)00350-8.
An alternative HPLC method for the quantification of the depolarizing neuromuscular blocking agent succinylcholine in human plasma is described. Drug spiked plasma and patient plasma samples were extracted using a C1 solid-phase cartridge. Succinylcholine was separated on a Cyano column and quantitated using electrochemical detection at a potential of 450 mV and 750 mV. Mobile phase consisted of a mixture of phosphoric acid-acetonitrile-methanol (45:35:25) adjusted to an apparent pH of 5. Standard curves for the quantitation were linear in the range of 250-8000 ng/ml. Between-day and within-day relative standard deviations were 5.1% and 1.7%, respectively. Mean drug recovery and accuracy was 68% and 104%, respectively.
本文描述了一种用于定量测定人血浆中去极化神经肌肉阻滞剂琥珀酰胆碱的高效液相色谱法。加标药物的血浆和患者血浆样本采用C1固相柱进行萃取。琥珀酰胆碱在氰基柱上分离，并采用电化学检测法在450 mV和750 mV电位下进行定量。流动相由磷酸 - 乙腈 - 甲醇（45:35:25）混合而成，调节至表观pH值为5。定量标准曲线在250 - 8000 ng/ml范围内呈线性。日间和日内相对标准偏差分别为5.1%和1.7%。药物平均回收率和准确度分别为68%和104%。
