Munakata R, Irié T, Cheng J, Nakajima T, Saku T
Department of Pathology, Nilgata University School of Dentistry, Japan.
J Oral Pathol Med. 1996 Sep;25(8):441-8. doi: 10.1111/j.1600-0714.1996.tb00294.x.
In order to reconstruct the characteristic three-dimensional architecture of adenoid cystic carcinoma, we cultured ACC2 cells, a cell system established from a human adenoid cystic carcinoma of the palate, in collagen gel matrix and transplanted them in SCID mice. In the collagen gel culture, the cells formed spherical colonies measuring 75.6 +/- 14.6 microns in diameter by 6 days after seeding. The tumor cell nests contained vacuolar structures that were immunopositive for heparan sulfate proteoglycan, type III collagen, type IV collagen, and fibronectin. The rim of the nests was argyrophilic and immunopositive for type I collagen, type IV collagen, laminin, and fibronectin. Transplants of ACC2 cells in SCID mice grew to form tumor masses in which pseudocysts were formed. The results indicate that our collagen gel culture system provides physiological conditions for ACC2 cells to secrete particular extracellular matrix molecules and form pseudocystic spaces.
为了重建腺样囊性癌的特征性三维结构，我们培养了ACC2细胞（一种从人腭部腺样囊性癌建立的细胞系），将其置于胶原凝胶基质中培养，然后移植到SCID小鼠体内。在胶原凝胶培养中，接种6天后细胞形成了直径为75.6±14.6微米的球形集落。肿瘤细胞巢含有对硫酸乙酰肝素蛋白聚糖、III型胶原、IV型胶原和纤连蛋白呈免疫阳性的空泡结构。巢的边缘对嗜银染色呈阳性，对I型胶原、IV型胶原、层粘连蛋白和纤连蛋白呈免疫阳性。将ACC2细胞移植到SCID小鼠体内后生长形成肿瘤块，其中形成了假囊肿。结果表明，我们的胶原凝胶培养系统为ACC2细胞分泌特定的细胞外基质分子并形成假囊性空间提供了生理条件。
