Cheng J, Irié T, Munakata R, Kimura S, Nakamura H, He R G, Lui A R, Saku T
Department of Pathology, Niigata University School of Dentistry, Japan.
Virchows Arch. 1995;426(6):577-86. doi: 10.1007/BF00192112.
The biosynthesis of basement membrane molecules and fibronectin was studied in vitro in the two different human cell systems (ACC2 and ACC3) established from adenoid cystic carcinomas (ACC) of the salivary gland using immunofluorescence and confocal microscopy. When cells were attached and spread on dishes, fine granular immunofluorescence for type IV collagen, laminin, heparan sulphate proteoglycan, entactin, and fibronectin first appeared diffusely in the cytoplasm, and then changed in aggregation of coarse granules in the perinuclear area. With formation of colonies, these signals were present in the extracellular space, initially in the basal aspect of attached cells and consequently in the lateral intercellular space. After the cells formed a confluent monolayer, extracellular signals started to decrease in inverse proportion to the reappearance of intracellular ones. The results indicate that the parenchymal cells of ACC synthesize these five extracellular matrix molecules, secrete them into the extracellular milieu and remodel the extracellular deposits. It is suggested that the characteristic stromal architecture of ACC, represented by stromal pseudocysts, results from their own secretion of the basement membrane molecules and fibronectin.
利用免疫荧光和共聚焦显微镜技术，在从涎腺腺样囊性癌（ACC）建立的两种不同人类细胞系（ACC2和ACC3）中，对体外基底膜分子和纤连蛋白的生物合成进行了研究。当细胞附着并铺展在培养皿上时，IV型胶原、层粘连蛋白、硫酸乙酰肝素蛋白聚糖、巢蛋白和纤连蛋白的细颗粒免疫荧光首先在细胞质中弥漫性出现，然后在核周区域变为粗颗粒聚集。随着菌落的形成，这些信号出现在细胞外空间，最初在附着细胞的基底方面，随后在细胞间的侧向空间。细胞形成汇合单层后，细胞外信号开始减少，与细胞内信号的再次出现成反比。结果表明，ACC的实质细胞合成这五种细胞外基质分子，将它们分泌到细胞外环境中并重塑细胞外沉积物。提示以基质假囊肿为代表的ACC特征性基质结构是由其自身分泌的基底膜分子和纤连蛋白所致。
