Ceccatelli S, Grandison L, Scott R E, Pfaff D W, Kow L M
Laboratory of Neurobiology and Behavior, Rockefeller University, New York, N.Y., USA.
Neuroendocrinology. 1996 Nov;64(5):357-63. doi: 10.1159/000127139.
Expression and estrogen regulation of the genes for nitric-oxide (NO)-synthesizing enzymes (NO synthase, NOS) were investigated by in situ hybridization. This study focused on regions of the hypothalamus that contain estrogen receptors and regulate specific neuroendocrine functions related to female sexual behavior and food intake, among others. Ovariectomized (OVX) rats were treated with vehicle or 3 micrograms/100 g estradiol benzoate (EB) for 7 days. Brains were sectioned and hybridized with antisense riboprobes for neuronal NOS, macrophage NOS and endothelial NOS. In the hypothalamus, mRNA was clearly detectable only for the neuronal NOS with the probes used. A strong hybridization signal was observed in the supraoptic paraventricular and ventromedial nuclei (SON, PVN and VMN, respectively). Quantitative analysis showed an increase in neuronal NOS mRNA in the VMN of the OVX rats treated with EB. The increase was mainly in the ventrolateral aspect of the VMN. No significant changes were observed in the hypothalamic SON and PVN. The data suggest that the expression of neuronal NOS mRNA in VMN can be regulated by estrogen.
通过原位杂交研究了一氧化氮（NO）合成酶（NO synthase，NOS）基因的表达及其雌激素调节作用。本研究聚焦于下丘脑含有雌激素受体且调节与雌性性行为和食物摄入等相关特定神经内分泌功能的区域。对去卵巢（OVX）大鼠用赋形剂或3微克/100克苯甲酸雌二醇（EB）处理7天。将大脑切片并与针对神经元型NOS、巨噬细胞型NOS和内皮型NOS的反义核糖探针杂交。在下丘脑中，仅用所用探针可清楚检测到神经元型NOS的mRNA。在视上核、室旁核和腹内侧核（分别为SON、PVN和VMN）观察到强杂交信号。定量分析显示，用EB处理的OVX大鼠VMN中神经元型NOS mRNA增加。增加主要在VMN的腹外侧部分。在下丘脑SON和PVN中未观察到显著变化。数据表明，VMN中神经元型NOS mRNA的表达可受雌激素调节。
