Revelo M P, Bambirra E A, Ferreira A P, Diniz C R, Chávez-Olórtegui C
General Pathology Department of the Biologic Science Institute (ICB), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Toxicon. 1996 Oct;34(10):1119-25. doi: 10.1016/0041-0101(96)00074-8.
In the present study we report the distribution of Tityus serrulatus scorpion venom in serum and various tissues of CFI mice and the efficacy of antivenom in reducing venom concentration. The animals were injected s.c. with 10 micrograms of scorpion venom, divided into groups of four animals and killed at different times from 15 min to 24 hr. Blood samples and samples of different tissues (heart, lung, liver, kidney, spleen, brain and injection site) were collected. Maximum venom levels occurred at 15 min in the kidney and liver and at 30 min in serum, lung, heart and spleen. After 2 hr the venom decreased rapidly in serum and in all other organs until venom levels were no longer detectable after 8 hr. No venom was detected in the central nervous system. In another experiment, 10 microliters of scorpion antivenom was injected i.v. together with the venom, and a rapid reduction of venom concentration was observed in the blood and tissues. In the third experiment, anti-scorpion venom was injected i.v. 1 hr after venom administration, and partial reduction of venom concentration was detected in tissues (lung and kidney). These studies contribute to the elaboration of more objective treatment that may result in a more economic, efficient and controlled use of scorpion antivenom in stings involving humans.
在本研究中，我们报告了锯齿脂鲤属蝎子毒液在CFI小鼠血清和各种组织中的分布情况，以及抗蛇毒血清在降低毒液浓度方面的功效。给动物皮下注射10微克蝎子毒液，将其分成每组四只动物，并在15分钟至24小时的不同时间处死。采集血样和不同组织（心脏、肺、肝脏、肾脏、脾脏、大脑和注射部位）的样本。毒液在肾脏和肝脏中15分钟时达到最高水平，在血清、肺、心脏和脾脏中30分钟时达到最高水平。2小时后，血清和所有其他器官中的毒液迅速下降，直到8小时后毒液水平不再可检测到。在中枢神经系统中未检测到毒液。在另一项实验中，静脉注射10微升蝎子抗蛇毒血清与毒液一起，观察到血液和组织中的毒液浓度迅速降低。在第三个实验中，在注射毒液1小时后静脉注射抗蝎子毒液，在组织（肺和肾脏）中检测到毒液浓度部分降低。这些研究有助于制定更客观的治疗方法，这可能会导致在涉及人类的蝎子蜇伤中更经济、有效和可控地使用蝎子抗蛇毒血清。
