一种新型吡啶核苷酸二硫化物氧化还原酶对于黄杆菌菌株Py2依赖NAD⁺和NADPH的环氧烷烃降解至关重要。
A novel type of pyridine nucleotide-disulfide oxidoreductase is essential for NAD+- and NADPH-dependent degradation of epoxyalkanes by Xanthobacter strain Py2.
作者信息
Swaving J, de Bont J A, Westphal A, de Kok A
机构信息
Division of Industrial Microbiology, Department of Food Science, Wageningen Agricultural University, The Netherlands.
出版信息
J Bacteriol. 1996 Nov;178(22):6644-6. doi: 10.1128/jb.178.22.6644-6646.1996.
Abstract
Epoxide degradation in cell extracts of Xanthobacter strain Py2 has been reported to be dependent on NAD+ and dithiols. This multicomponent system has now been fractionated. A key protein encoded by a DNA fragment complementing a Xanthobacter strain Py2 mutant unable to degrade epoxides was purified and analyzed. This NADP-dependent protein, a novel type of pyridine nucleotide-disulfide oxidoreductase, is essential for epoxide degradation. NADPH, acting as the physiological cofactor, replaced the dithiols in epoxide conversion.
摘要
据报道，黄色杆菌属菌株Py2细胞提取物中的环氧化物降解依赖于NAD⁺和二硫醇。现在已对该多组分系统进行了分级分离。对一个由DNA片段编码的关键蛋白进行了纯化和分析，该DNA片段可互补一株无法降解环氧化物的黄色杆菌属菌株Py2突变体。这种依赖NADP的蛋白是一种新型的吡啶核苷酸 - 二硫键氧化还原酶，对环氧化物降解至关重要。作为生理辅因子的NADPH在环氧化物转化中取代了二硫醇。
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9
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