检测乳腺癌患者淋巴结微转移的角蛋白19信使核糖核酸测量
Keratin 19 mRNA measurement to detect micrometastases in lymph nodes in breast cancer patients.
作者信息
Schoenfeld A, Luqmani Y, Sinnett H D, Shousha S, Coombes R C
机构信息
Department of Surgery, Charing Cross Hospital, London, UK.
出版信息
Br J Cancer. 1996 Nov;74(10):1639-42. doi: 10.1038/bjc.1996.601.
Abstract
We have used polymerase chain reaction (PCR) to measure keratin 19 mRNA in order to detect breast cancer cells invading axillary lymph nodes. In a consecutive series of 125 patients with primary breast cancer, 75 patients had no evidence of lymph node involvement by conventional histology. A total of 530 lymph nodes from these patients were examined and 106 (20%) gave a keratin 19 product detectable by Southern hybridisation. This correlated with primary tumour size (P<0.001). These 106 nodes came from 23 patients. Thus, using this technique, 23/75 (30.6%) patients were found to have evidence of lymph node involvement who would otherwise have been designated lymph node negative.
摘要
我们采用聚合酶链反应（PCR）来检测角蛋白19信使核糖核酸（mRNA），以发现侵袭腋窝淋巴结的乳腺癌细胞。在连续的125例原发性乳腺癌患者中，75例患者经传统组织学检查未发现淋巴结受累迹象。对这些患者的总共530个淋巴结进行了检查，其中106个（20%）通过Southern杂交检测到角蛋白19产物。这与原发肿瘤大小相关（P<0.001）。这106个淋巴结来自23例患者。因此，运用这项技术，发现23/75（30.6%）的患者有淋巴结受累迹象，否则这些患者会被认定为淋巴结阴性。
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