酿酒酵母IRE2/HAC1参与IRE1介导的KAR2表达。
Saccharomyces cerevisiae IRE2/HAC1 is involved in IRE1-mediated KAR2 expression.
作者信息
Nikawa J, Akiyoshi M, Hirata S, Fukuda T
机构信息
Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.
出版信息
Nucleic Acids Res. 1996 Nov 1;24(21):4222-6. doi: 10.1093/nar/24.21.4222.
Abstract
The Saccharomyces cerevisiae IRE1 gene, encoding a putative receptor-type protein kinase, is known to be required for inositol prototrophy and for the induction of a chaperon molecule, BiP, encoded by KAR2, under stress conditions such as tunicamycin addition. We have characterized a yeast gene, IRE2, which was isolated as a suppressor gene that complements the inositol auxotrophic phenotype of the ire1 mutation. Sequencing analysis revealed that IRE2 is identical to HAC1, which encodes a transcription factor having a basic-leucine zipper motif. Introduction of IRE2/HAC1 into the ire1 mutant clearly restored the expression of KAR2 upon tunicamycin treatment. ire2/hac1-disrupted yeast cells showed not only the inositol auxotrophic phenotype but also the tunicamycin sensitivity, and failed to induce the expression of KAR2. These results clearly indicate that the IRE2/HAC1 gene product plays a critical role in the induction of KAR2 expression and in the inositol prototrophy mediated by IRE1.
摘要
酿酒酵母IRE1基因编码一种假定的受体型蛋白激酶，已知在添加衣霉素等应激条件下，该基因对于肌醇原养型以及诱导由KAR2编码的伴侣分子BiP是必需的。我们鉴定了一个酵母基因IRE2，它是作为一个抑制基因分离出来的，可弥补ire1突变的肌醇营养缺陷型表型。测序分析表明，IRE2与HAC1相同，HAC1编码一种具有碱性亮氨酸拉链基序的转录因子。将IRE2/HAC1导入ire1突变体后，在衣霉素处理时明显恢复了KAR2的表达。ire2/hac1破坏的酵母细胞不仅表现出肌醇营养缺陷型表型，还表现出对衣霉素的敏感性，并且无法诱导KAR2的表达。这些结果清楚地表明，IRE2/HAC1基因产物在KAR2表达的诱导以及由IRE1介导的肌醇原养型中起关键作用。
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