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Envelope glycoproteins from biologically diverse isolates of immunodeficiency viruses have widely different affinities for CD4.来自免疫缺陷病毒生物学上不同分离株的包膜糖蛋白对CD4具有广泛不同的亲和力。
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7
A new cationic liposome reagent mediating nearly quantitative transfection of animal cells.一种新型阳离子脂质体试剂，可介导动物细胞近乎定量的转染。
Biotechniques. 1991 Apr;10(4):520-5.

一种高效的二甲基二烯丙基溴化铵介导的果蝇S2细胞转染方法。

An efficient DDAB-mediated transfection of Drosophila S2 cells.

作者信息

Han K

机构信息

Department of Genetic Engineering, Hallym University, Chunchon, Republic of Korea.

出版信息

Nucleic Acids Res. 1996 Nov 1;24(21):4362-3. doi: 10.1093/nar/24.21.4362.

DOI:10.1093/nar/24.21.4362
PMID:8932397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146234/
Abstract

I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.

摘要

我已经开发出一种使用阳离子脂质体试剂二油酰基二甲基溴化铵（DDAB）转染果蝇S2细胞的有效方法。与传统的磷酸钙方法相比，优化后的DDAB方法使转染效率提高了10倍或更多，而磷酸钙方法基本上是转染S2细胞的唯一方法。