LILAC：利用光遗传学 Lifeact 增强肌动蛋白成像。
LILAC: enhanced actin imaging with an optogenetic Lifeact.
机构信息
Biophysical Sciences Graduate Program, The University of Chicago, Chicago, IL, USA.
Neuroscience Program, University of Illinois Urbana-Champaign, Urbana, IL, USA.
出版信息
Nat Methods. 2023 Feb;20(2):214-217. doi: 10.1038/s41592-022-01761-3. Epub 2023 Jan 30.
Abstract
Lifeact is a popular peptide-based label of actin filaments in live cells. We have designed an improved Lifeact variant, LILAC, that binds to actin in light using the LOV2 protein. Light control allows the user to modulate actin labeling, enabling image analysis that leverages modulation for an enhanced view of F-actin dynamics in cells. Furthermore, the tool reduces actin perturbations and cell sickness caused by Lifeact overexpression.
摘要
Lifeact 是一种在活细胞中标记肌动蛋白丝的常用肽基标签。我们设计了一种改良的 Lifeact 变体 LILAC，它使用 LOV2 蛋白在光照下与肌动蛋白结合。光照控制允许用户调节肌动蛋白标记，从而进行图像分析，利用调制增强细胞中 F-actin 动力学的观察效果。此外，该工具还减少了因 Lifeact 过表达而引起的肌动蛋白扰动和细胞病变。
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