Mahanty S, Luke H E, Kumaraswami V, Narayanan P R, Vijayshekaran V, Nutman T B
Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA.
Exp Parasitol. 1996 Nov;84(2):282-90. doi: 10.1006/expr.1996.0114.
Parasite stage-specific T cell responses were studied in Indians with lymphatic filariasis manifesting as elephantiasis (CP, n = 11) and asymptomatic microfilaremia (MF, n = 8), using antigens derived from the microfilarial, adult male only, and mixed adult male and female worms. Proliferative responses to microfilarial and mixed (male-female adult worm) antigens in MF individuals were markedly impaired compared to corresponding responses in individuals with CP. In contrast, T cell proliferative responses to adult male-derived antigens were not statistically different between the two groups. Analysis of antigen-driven cytokine secretion by peripheral blood mononuclear cells from MF and CP individuals revealed significantly lower IL-2 and IFN-gamma production by MF in response to microfilarial and mixed antigens (but not to adult male antigen) compared to CP individuals. No differences were observed between MF and CP in parasite antigen-driven IL-4 or IL-5 production. Spontaneous and parasite-specific IL-10 secretion was also measured to determine if cytokine cross-regulation of Th1 responses may be a mechanism underlying the observed Th1 suppression. Spontaneous and microfilarial antigen-driven IL-10 was found to be significantly higher in MF than in CP individuals. These data indicate that MF individuals exhibit preferentially impaired Th1-type responses to microfilarial antigens and that microfilarial-induced IL-10 may be critical in the downregulation of specific Th1 responses.
利用源自微丝蚴、仅成年雄虫以及成年雄虫和雌虫混合体的抗原，对表现为象皮肿的淋巴丝虫病印度患者（CP组，n = 11）和无症状微丝蚴血症患者（MF组，n = 8）的寄生虫阶段特异性T细胞反应进行了研究。与CP组个体的相应反应相比，MF组个体对微丝蚴和混合（成年雄虫和雌虫）抗原的增殖反应明显受损。相比之下，两组之间对成年雄虫来源抗原的T细胞增殖反应无统计学差异。对MF组和CP组个体外周血单个核细胞的抗原驱动细胞因子分泌分析显示，与CP组个体相比，MF组个体在对微丝蚴和混合抗原（而非成年雄虫抗原）的反应中，IL-2和IFN-γ的产生显著降低。在寄生虫抗原驱动的IL-4或IL-5产生方面，未观察到MF组和CP组之间存在差异。还检测了自发和寄生虫特异性IL-10分泌，以确定Th1反应的细胞因子交叉调节是否可能是观察到的Th1抑制的潜在机制。发现MF组个体中自发和微丝蚴抗原驱动的IL-10显著高于CP组个体。这些数据表明，MF组个体对微丝蚴抗原的Th1型反应优先受损，并且微丝蚴诱导的IL-10可能在特异性Th1反应的下调中起关键作用。
