Pinkerton A C, O'Brochta D A, Atkinson P W
Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australia.
Insect Mol Biol. 1996 Nov;5(4):223-7. doi: 10.1111/j.1365-2583.1996.tb00096.x.
The P, hobo, Hermes, Minos and mariner transposable elements have been successfully used as gene vectors to achieve genetic transformation of Drosophila melanogaster. The hobo transposable element of D. melanogaster, a member of the hAT family of transposable elements, can also transpose in other dipteran species including the house fly Musca domestica and the Queensland fruit fly, Bactrocera tryoni. Another hAT element, Hermes, can also transpose in a wide range of insect species. We report here that both of these hAT elements are also capable of mobility and accurate transposition in the noctuid Helicoverpa armigera and thus may be able to be used as gene vectors that will enable the genetic transformation of this important pest species. Significantly, the ability of hobo to excise in this species is independent of the presence of an exogenous source of hobo transposase, which suggests that H. armigera may possess an endogenous hobo-like transposable element.
P、hobo、Hermes、Minos和水手转座元件已成功用作基因载体，以实现黑腹果蝇的遗传转化。黑腹果蝇的hobo转座元件是hAT转座元件家族的成员，它也能在包括家蝇和昆士兰果蝇在内的其他双翅目物种中发生转座。另一个hAT元件Hermes也能在多种昆虫物种中发生转座。我们在此报告，这两种hAT元件在夜蛾棉铃虫中也具有移动性和精确转座能力，因此有可能用作基因载体，实现对这种重要害虫物种的遗传转化。值得注意的是，hobo在该物种中切除的能力不依赖于外源hobo转座酶的存在，这表明棉铃虫可能拥有一种内源性的类似hobo的转座元件。
