O'Brochta D A, Atkinson P W
Center for Agricultural Biotechnology, University of Maryland, College Park 20742, USA.
Insect Biochem Mol Biol. 1996 Sep-Oct;26(8-9):739-53. doi: 10.1016/s0965-1748(96)00022-7.
This review summarizes recent data on the development of non-drosophilid insect transformation systems. The discussion focuses on one particular approach to developing transformation systems that relies on the use of short inverted repeat-type transposable elements analogous to that employed for Drosophila melanogaster transformation. Representatives from four families of short inverted repeat-type transposable elements have been shown to either act as non-drosophilid gene vectors or to have the ability to transpose accurately when introduced into non-host insect cells. Minos, a member of the Tcl family of elements isolated originally from D. hydei has been successfully used as a germline transformation vector in the Medfly, Ceratitis capitata. Hermes, a member of the hAT family of elements isolated originally from Musca domestica has been successfully used as a gene transformation vector in D. melanogaster and has a host range that appears to include culicids. hobo, another member of the hAT family of elements isolated from D. melanogaster also has a broad host range that includes tephritid fruitflies. mariner(Mos), a member of the mariner family of elements isolated from D. mauritiana can transpose in calliphorids. Finally, piggyBac/IFP2, a member of the TTAA-specific family of elements isolated from Trichoplusia ni can transpose when introduced into Spodoptera frugiperda cells. Although routine transformation of insects other than D. melanogaster is not possible it is clear that the raw materials for the development of efficient transformation systems are now available.
本综述总结了关于非果蝇类昆虫转化系统发展的最新数据。讨论聚焦于一种开发转化系统的特定方法，该方法依赖于使用与用于黑腹果蝇转化的短反向重复型转座元件类似的元件。已证明来自四个短反向重复型转座元件家族的代表要么可作为非果蝇类基因载体，要么在引入非宿主昆虫细胞时具有准确转座的能力。米诺斯（Minos）是最初从海德氏果蝇（D. hydei）分离的Tcl元件家族的成员，已成功用作地中海实蝇（Ceratitis capitata）的种系转化载体。赫耳墨斯（Hermes）是最初从家蝇（Musca domestica）分离的hAT元件家族的成员，已成功用作黑腹果蝇的基因转化载体，其宿主范围似乎包括蚊科昆虫。霍博（hobo）是另一个从黑腹果蝇分离的hAT元件家族的成员，其宿主范围也很广，包括实蝇科果蝇。水手（mariner，Mos）是从毛里求斯果蝇（D. mauritiana）分离的mariner元件家族的成员，可在丽蝇科昆虫中转座。最后，从粉纹夜蛾（Trichoplusia ni）分离的TTAA特异性元件家族的成员piggyBac/IFP2在引入草地贪夜蛾（Spodoptera frugiperda）细胞时可转座。虽然除黑腹果蝇外的昆虫进行常规转化尚不可能，但显然现在已有开发高效转化系统的原材料。
