饲料中乙氧酰胺苯甲酯的高压液相色谱测定法
High pressure liquid chromatographic determination of ethopabate in feeds.
作者信息
Schronk L R, Colvin B M, Hanks A R, Bushway R J
出版信息
J Assoc Off Anal Chem. 1977 Sep;60(5):1048-50.
A high pressure liquid chromatographic (HPLC) method is described for determining ethopabate in poultry feeds. Feed samples containing ethopabate are finely ground, extracted 30 min by sonification with methanol-water (80 + 20), and filtered. The extract is cleaned up on an alumina column, and the first 4 ml eluate collected is analyzed. The drug is eluted through a muBondapak C18 column with acetonitrile-water (30 + 70) at a flow rate of 1.4 ml/min and detected by ultraviolet absorption at 280 nm. Chromatography is complete in 7 min, and detector response is linear. The drug is quantitated by peak height ratios. The procedure described is reproducible and shows good agreement with the official AOAC colorimetric method. The lower limit of detection is 2 ng by HPLC, making the method applicable to residue analyses.
本文描述了一种用于测定家禽饲料中乙氧酰胺苯甲酯的高压液相色谱（HPLC）方法。含有乙氧酰胺苯甲酯的饲料样品经精细研磨后，用甲醇 - 水（80 + 20）超声提取30分钟，然后过滤。提取物在氧化铝柱上进行净化处理，收集最初的4毫升洗脱液进行分析。该药物通过μBondapak C18柱，以乙腈 - 水（30 + 70）为流动相，流速为1.4毫升/分钟进行洗脱，并在280纳米处通过紫外吸收进行检测。7分钟内完成色谱分析，检测器响应呈线性。通过峰高比进行药物定量。所描述的方法具有可重复性，并且与官方AOAC比色法结果吻合良好。HPLC法的检测下限为2纳克，该方法适用于残留分析。
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