Rauha J P, Salomies H, Aalto M
Department of Pharmacy, University of Helsinki, Finland.
J Pharm Biomed Anal. 1996 Nov;15(2):287-93. doi: 10.1016/0731-7085(96)01846-8.
Liquid chromatographic methods were developed for the determination of bromhexine hydrochloride, methyl p-hydroxybenzoate and propyl p-hydroxybenzoate (method A) and dextromethorphan hydrobromide (method B) in cough-cold syrup formulations. Reversed-phase analytical columns (150 mm x 3.9 mm i.d.) were used with (A) C18 and (B) phenyl as stationary phases and mixtures of (A) acetonitrile and aqueous 15 mM triethylamine solution (43:57) and (B) methanol and aqueous 3% ammonium formate buffer solution (53:47) as mobile phases at a flow rate of 1.0 ml min-1. Both aqueous components were adjusted to pH 3.9. UV detection of analytes was at (A) 245 nm and (B) 278 nm. In both methods, the time required for an HPLC run giving good separations and recoveries was less than 8 min.
已开发出液相色谱法，用于测定止咳感冒糖浆制剂中的盐酸溴己新、对羟基苯甲酸甲酯和对羟基苯甲酸丙酯（方法A）以及氢溴酸右美沙芬（方法B）。使用反相分析柱（内径150 mm×3.9 mm），（A）以C18为固定相，（B）以苯基为固定相，（A）以乙腈和15 mM三乙胺水溶液（43:57）的混合物以及（B）以甲醇和3%甲酸铵缓冲水溶液（53:47）为流动相，流速为1.0 ml min-1。两种水性组分均调节至pH 3.9。分析物的紫外检测波长为（A）245 nm和（B）278 nm。在两种方法中，实现良好分离和回收率的高效液相色谱运行所需时间均少于8分钟。
