Kone B C
Department of Internal Medicine, University of Texas Health Science Center at Houston 77030, USA.
Miner Electrolyte Metab. 1996;22(5-6):349-65.
The H,K-ATPase comprises a family of isoenzymes with unique biochemical, pharmacological, and regulatory properties. This review explores recent advances in discovery of the molecular biology, biochemistry, and function of these ion pumps, with particular emphasis on the implications for renal potassium and proton handling. Structure-function correlations governing the ion transport mechanisms, inhibitor binding sites, oligomerization, and transcriptional control of the H,K-ATPase isoforms are examined. Functional studies of H,K-ATPase in renal tubules and cultured epithelial cells are analyzed and related to data concerning the expression and distribution of the H,K-ATPase gene products in the kidney. Functional and molecular biological evidence for adaptive changes in renal H,K-ATPase expression during the evolution of potassium and acid-base disturbances are discussed. Current investigative challenges and avenues for future research of these enzymes are presented.
H，K - ATP酶由具有独特生化、药理和调节特性的同工酶家族组成。本综述探讨了这些离子泵在分子生物学、生物化学和功能发现方面的最新进展，特别强调了其对肾脏钾离子和质子处理的影响。研究了控制H，K - ATP酶同工型离子转运机制、抑制剂结合位点、寡聚化和转录调控的结构 - 功能关系。分析了肾小管和培养上皮细胞中H，K - ATP酶的功能研究，并将其与肾脏中H，K - ATP酶基因产物的表达和分布数据相关联。讨论了钾离子和酸碱平衡紊乱演变过程中肾脏H，K - ATP酶表达适应性变化的功能和分子生物学证据。介绍了目前这些酶的研究挑战和未来研究方向。
