Yousfi M M, el-Zimaity H M, Genta R M, Graham D Y
Department of Medicine, Veterans Affairs Medical Center, Houston, Texas 77030, USA.
Gastrointest Endosc. 1996 Nov;44(5):519-22. doi: 10.1016/s0016-5107(96)70001-9.
Rapid urease tests are commonly used as a convenient method to detect Helicobacter pylori infection. Our previous experiments demonstrated enhanced efficacy of agar gel rapid urease test compared with reagent strip rapid urease tests. We evaluated the efficacy of PyloriTek, a new reagent strip rapid test for detecting H. pylori infection.
Gastric antral mucosal biopsy specimens were obtained for comparison between agar gel rapid urease tests and PyloriTek (200 specimens). The rapid urease test to be used first was selected randomly. H. pylori status was determined using the Genta stain. Culture was performed to confirm H. pylori status when false rapid urease tests were suspected.
One hundred patients were studied; 68 had H. pylori infection. There were two false-negative and one false-positive PyloriTek when scored at 1 hour, compared with only one false-positive and no false-negative tests at 2 hours. With the agar gel rapid urease tests, there were no false-positive tests and 5 false-negative tests when scored at 1 hour, 2 false-negative tests at 12 hours and 1 at 24 hours; there were no false-positive tests. At 1 hour, 3% (95% CI = 1% to 9%) of PyloriTek tests had an erroneous categorization of H. pylori status compared with 5% for the agar gel rapid urease tests (95% CI = 1.6% to 11%) (p > 0.7).
The new reagent strip rapid urease test, PyloriTek, is rapid and comparable in accuracy to agar gel rapid urease tests for detecting H. pylori Infection.
快速尿素酶试验是检测幽门螺杆菌感染的常用便捷方法。我们之前的实验表明，与试剂条快速尿素酶试验相比，琼脂凝胶快速尿素酶试验的效果更佳。我们评估了一种新型试剂条快速检测法PyloriTek检测幽门螺杆菌感染的效果。
获取胃窦黏膜活检标本，用于琼脂凝胶快速尿素酶试验和PyloriTek（200份标本）之间的比较。首先使用的快速尿素酶试验随机选择。使用金塔染色法确定幽门螺杆菌状态。当怀疑快速尿素酶试验结果为假阳性时，进行培养以确认幽门螺杆菌状态。
对100名患者进行了研究；其中68人感染了幽门螺杆菌。PyloriTek在1小时评分时有2例假阴性和1例假阳性，而在2小时评分时只有1例假阳性且无假阴性试验。对于琼脂凝胶快速尿素酶试验，1小时评分时无假阳性试验，有5例假阴性试验；12小时时有2例假阴性试验，24小时时有1例假阴性试验；无假阳性试验。在1小时时，3%（95%CI=从1%至9%）的PyloriTek试验对幽门螺杆菌状态的分类有误，而琼脂凝胶快速尿素酶试验为5%（95%CI=1.6%至11%）（p>0.7）。
新型试剂条快速尿素酶试验PyloriTek在检测幽门螺杆菌感染方面速度快，准确性与琼脂凝胶快速尿素酶试验相当。
