Young E L, Sharma T K, Cutler A F
Section of Gastroenterology, Sinai Hospital, Detroit, Michigan 48235, USA.
Gastrointest Endosc. 1996 Nov;44(5):527-31. doi: 10.1016/s0016-5107(96)70003-2.
To determine the sensitivity, specificity, and positive and negative predictive values of a newly developed urea-membrane test for the detection of Helicobacter pylori in gastric tissue.
Patients presenting for upper endoscopy with no recent exposure to H. pylori-altering drugs were enrolled. Antral biopsy specimens were tested by the urea-membrane and urea-gel methods and submitted for histology. Patients underwent [13C]urea breath tests. Presence of H. pylori was established by histology or the combination of a positive [13C]urea breath test and a positive urea-gel test. Absence of H.pylori required both the [13C]urea breath test and the invasive tests to be negative. The urea-membrane test was reported at 1 hour.
Ninety-nine patients (47 men and 52 women) with a mean age of 51.43 +/- 14.9 years participated. Fifty of 99 patients (prevalence, 50.5%) tested positive for H. pylori. The urea-membrane test correctly identified 49 of 50 H. pylori-positive and 46 of 49 H. pylori-negative patients, yielding sensitivity, specificity, and positive and negative predictive values of 98.0%, 93.9%, and 94.2% and 97.9%, respectively, in this population.
Rapidly available and reliable results from the urea-membrane test can facilitate clinical decision prior to patient discharge from the endoscopy suite.
确定一种新开发的尿素膜试验检测胃组织中幽门螺杆菌的敏感性、特异性、阳性预测值和阴性预测值。
纳入近期未接触过改变幽门螺杆菌药物的接受上消化道内镜检查的患者。对胃窦活检标本采用尿素膜法和尿素凝胶法进行检测，并送检组织学检查。患者接受[13C]尿素呼气试验。通过组织学检查或[13C]尿素呼气试验阳性与尿素凝胶试验阳性相结合来确定幽门螺杆菌的存在。幽门螺杆菌阴性则要求[13C]尿素呼气试验和侵入性试验均为阴性。尿素膜试验在1小时后报告结果。
99例患者（47例男性和52例女性）参与研究，平均年龄为51.43±14.9岁。99例患者中有50例（患病率为50.5%）幽门螺杆菌检测呈阳性。尿素膜试验正确识别出50例幽门螺杆菌阳性患者中的49例以及49例幽门螺杆菌阴性患者中的46例，在该人群中，其敏感性、特异性、阳性预测值和阴性预测值分别为98.0%、93.9%、94.2%和97.9%。
尿素膜试验快速可得且结果可靠，有助于在内镜检查室患者出院前做出临床决策。
