Bhat T K, Makkar H P, Singh B
Regional Station, Indian Veterinary Research Institute, Palampur, India.
Lett Appl Microbiol. 1996 Apr;22(4):257-8. doi: 10.1111/j.1472-765x.1996.tb01155.x.
Faecal samples of 52 hill cattle fed largely on oak leaves were screened for tannin-protein complex-degrading micro-organisms under different culture conditions using tannin-treated brain heart infusion agar medium. None of the animals were found to harbour tannin-protein complex-degrading enterobacteria. However, a fungus identified as Aspergillus niger van Tieghem having tannin-protein complex-degrading activity, was consistently isolated from the faeces of such animals. Optimum growth and sporulation was noticed under aerobic conditions at 30 degrees C on Czapek Yeast Agar medium.
在不同培养条件下,使用经单宁处理的脑心浸液琼脂培养基,对52头主要以橡树叶为食的山地牛的粪便样本进行筛选,以寻找能降解单宁 - 蛋白质复合物的微生物。未发现任何动物体内存在能降解单宁 - 蛋白质复合物的肠杆菌。然而,一种被鉴定为黑曲霉(Aspergillus niger van Tieghem)且具有单宁 - 蛋白质复合物降解活性的真菌,一直从这些动物的粪便中分离出来。在30摄氏度有氧条件下,察贝克酵母琼脂培养基上观察到其最佳生长和孢子形成。