Takayanagi S, Kawasaki H, Sugimori K, Yamada T, Sugai A, Ito T, Yamasato K, Shioda M
Department of Biology, Toho University School of Medicine, Ota-ku, Tokyo, Japan.
Int J Syst Bacteriol. 1996 Apr;46(2):377-82. doi: 10.1099/00207713-46-2-377.
We characterized a microbial strain that was isolated from a hot spring at a geothermal area in Hakone, Japan. This isolate, whose lobed-shaped cells were about 1.0 micron in diameter, was a facultative chemolitho-autotroph that required aerobic conditions for growth. The optimum pH was 3.0 (pH range, 1.0 to 4.0), and the optimum temperature was 70 degrees C (temperature range, 50 to 80 degrees C). Lithotrophically, this strain grew on elemental sulfur and reduced sulfur compounds. The G+C content of the genomic DNA was 38.4 mol%. This organism contained calditoglycerocaldarchaeol, which is characteristic of members of the Sulfolobaceae. The levels of 16S rRNA sequence similarity between the new isolate and Sulfolobus acidocaldarius, Sulfolobus solfataricus, and Sulfolobus shibatae were less than 89.8%. Unlike S. acidocaldarius, S. solfataricus, and S. shibatae, the new isolate utilized sugars and amino acids poorly as sole carbon sources, and the levels of DNA-DNA hybridization between the new isolate and these Sulfolobus species were very low. Phenotypically, the new isolate was also distinct from the obligately lithotrophic organism Sulfolobus metallicus. We concluded that the new organism belongs to a new Sulfolobus species, for which we propose the name Sulfolobus hakonensis.
我们对从日本箱根地热区的一处温泉中分离出的一株微生物菌株进行了特性分析。该分离株的叶状细胞直径约为1.0微米，是一种兼性化能自养生物，生长需要有氧条件。最适pH为3.0（pH范围为1.0至4.0），最适温度为70℃（温度范围为50至80℃）。在化能营养方面，该菌株以元素硫和还原态硫化合物为生长底物。基因组DNA的G+C含量为38.4摩尔%。该生物含有钙甘油古菌醇，这是硫化叶菌科成员的特征。新分离株与嗜酸硫化叶菌、嗜热栖热硫化叶菌和柴田硫化叶菌之间的16S rRNA序列相似性水平低于89.8%。与嗜酸硫化叶菌、嗜热栖热硫化叶菌和柴田硫化叶菌不同，新分离株作为唯一碳源利用糖类和氨基酸的能力较差，并且新分离株与这些硫化叶菌物种之间的DNA-DNA杂交水平非常低。从表型上看，新分离株也与专性化能自养生物金属硫化叶菌不同。我们得出结论，该新生物属于一个新的硫化叶菌物种，我们提议将其命名为箱根硫化叶菌。
