Panagakos F S, O'Boskey J F, Rodriguez E
Department of Prosthodontics and Biomaterials, New Jersey Dental School, Newark 07103, USA.
J Endod. 1996 Jul;22(7):358-61. doi: 10.1016/S0099-2399(96)80218-4.
Little information is currently known regarding the effects of cytokines and lipopolysaccharides (LPS's) on matrix metalloproteinase (MMP) production by pulp cells in vitro. In this study, human pulp cells (HPC's) and clonal rat pulp cells RPC-C2A were treated with interleukin (IL)-1 alpha, IL-1 beta, tumor necrosis factor (TNF)-alpha, and LPS for 24 h. Conditioned medium and cell lysates were collected and analyzed by gelatin zymography. RPC-C2A cells treated with IL-1 beta and TNF-alpha displayed elevated levels of MMP's in conditioned medium fractions. LPS's at increasing concentrations had a similar effect. HPC's treated with either cytokines or LPS's had no change in the pattern of MMP's produced or secreted in either cellular or conditioned medium fractions. These studies indicate that the effects of cytokines and LPS's on pulp cells are not identical for cells from different species and requires further investigation to clarify these variations.
目前关于细胞因子和脂多糖（LPS）对体外牙髓细胞产生基质金属蛋白酶（MMP）的影响所知甚少。在本研究中，人牙髓细胞（HPC）和克隆大鼠牙髓细胞RPC-C2A用白细胞介素（IL）-1α、IL-1β、肿瘤坏死因子（TNF）-α和LPS处理24小时。收集条件培养基和细胞裂解物，并通过明胶酶谱法进行分析。用IL-1β和TNF-α处理的RPC-C2A细胞在条件培养基组分中显示出MMP水平升高。浓度不断增加的LPS有类似的作用。用细胞因子或LPS处理的HPC在细胞或条件培养基组分中产生或分泌的MMP模式没有变化。这些研究表明，细胞因子和LPS对牙髓细胞的影响因细胞来源的物种不同而不同，需要进一步研究以阐明这些差异。
