Coil Jeffrey, Tam Esther, Waterfield J Douglas
Department of Oral, Biological, and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada.
J Endod. 2004 Feb;30(2):88-91. doi: 10.1097/00004770-200402000-00006.
Pulpal disease is intimately associated with the immune system's response to bacteria products. Clinical pathology is mediated in part by the production of pyrogenic cytokines, especially interleukin (IL)-1, tumor necrosis factor (TNF)-alpha, and IL-6. Methyl mercaptan (CH3SH), a volatile sulfur compound produced by anaerobic Gram-negative bacteria, has been shown to contribute to the production of IL-1 by human mononuclear cells. In this report, we investigated the production of IL-1, TNF-alpha, and IL-6 by human pulp fibroblasts when stimulated for various periods of time with lipopolysaccharide (LPS) with or without the presence of CH3SH. We found that LPS and CH3SH had no effect on the production of IL-1 or TNF-alpha. However, LPS stimulated IL-6 production, and this production was augmented when CH3SH was present. We conclude that the volatile sulfur compound CH3SH plays a role in activation and modulation of the immune response through its role in production of IL-6.
牙髓病与免疫系统对细菌产物的反应密切相关。临床病理学部分是由致热细胞因子的产生介导的，尤其是白细胞介素（IL）-1、肿瘤坏死因子（TNF）-α和IL-6。甲硫醇（CH3SH）是一种由厌氧革兰氏阴性菌产生的挥发性硫化合物，已被证明可促进人单核细胞产生IL-1。在本报告中，我们研究了人牙髓成纤维细胞在有或没有CH3SH存在的情况下，用脂多糖（LPS）刺激不同时间后IL-1、TNF-α和IL-6的产生。我们发现LPS和CH3SH对IL-1或TNF-α的产生没有影响。然而，LPS刺激了IL-6的产生，当存在CH3SH时，这种产生会增加。我们得出结论，挥发性硫化合物CH3SH通过其在IL-6产生中的作用，在免疫反应的激活和调节中发挥作用。
