Malicka-Błaszkiewicz M, Styczeń I, Nowak D, Hańczycowa H, Ponikowski P, Sebzda T
Institute of Biochemistry, University of Wrocław, Poland.
Mater Med Pol. 1995 Jul-Sep;27(3):115-8.
Monomeric G-actin, total actin and filamentous F-actin were examined during the growth of experimental tumour hepatoma Morris 5123. Actin was measured by the inhibition of the standard DNase I from bovine pancreas. A remarkable increase in total actin, and F-actin content, as well as in the state of actin polymerization (measured by the F:G actin ratio) was shown in the cytosol of the tumour cells in the second week of the tumour growth, followed by a rapid decrease in the third week. Parallel observations were made in the cytosol of the liver and in the serum of the tumour bearing rats. The results were compared with the data obtained for control healthy rats. It was shown that hepatoma Morris tumour growth is accompanied by the changes in the actin content and polymerization, occurring also in the liver of the host animal. Only G-actin was found in the serum. It increased significantly in the second week of tumour growth as compared with the G-actin level in the serum of the control healthy rats.
在实验性肿瘤——莫里斯5123肝癌生长过程中，对单体G-肌动蛋白、总肌动蛋白和丝状F-肌动蛋白进行了检测。通过抑制来自牛胰腺的标准脱氧核糖核酸酶I来测定肌动蛋白。在肿瘤生长的第二周，肿瘤细胞胞质溶胶中总肌动蛋白、F-肌动蛋白含量以及肌动蛋白聚合状态（通过F:G肌动蛋白比率测量）显著增加，随后在第三周迅速下降。对荷瘤大鼠的肝脏胞质溶胶和血清进行了平行观察。将结果与对照健康大鼠的数据进行了比较。结果表明，莫里斯肝癌的生长伴随着肌动蛋白含量和聚合的变化，宿主动物肝脏中也会出现这种变化。血清中仅发现G-肌动蛋白。与对照健康大鼠血清中的G-肌动蛋白水平相比，在肿瘤生长的第二周其显著增加。
