Andrzejewska A, Szynaka B, Stokowska W, Szynaka P
Mater Med Pol. 1995 Apr-Jun;27(2):43-6.
The aim of the paper is to make an ultrastructural analysis of the nuclei of the pancreatic acinar cells in the rat in the course of lead acetate intoxication. The experiment used 84 Wistar rats, divided into 3 experimental groups, and a control group. The experimental rats were given aqueous solution of lead acetate in a dose of 50 mg/dm3 in group I, 500 mg/dm3 in group II and 1000 mg/dm3 in group III. The control rats were given tap water. The animals were killed 2,4 and 6 weeks after the experiment. The pancreatic tissue was collected for ultrastructural analysis and lead level was determined in the blood and in dry pancreatic mass in an atomic absorption spectrometer. After 6 weeks of lead acetate administration the levels increased 5 to 10-fold compared with the control group. It was ultrastructurally demonstrated that a low dose of lead caused chromatin margination, extension of perinuclear space and folding of acinar cell nucleus contours. Chronic administration of high lead doses resulted in the formation of pseudoinclusions and intranuclear real inclusions of the lead-protein complex. The alterations provided evidence of the toxic effect of lead upon the acinar cells nuclei in the pancreas.
本文旨在对醋酸铅中毒过程中大鼠胰腺腺泡细胞核进行超微结构分析。实验使用了84只Wistar大鼠，分为3个实验组和1个对照组。实验组大鼠分别给予剂量为50mg/dm³的醋酸铅水溶液（I组）、500mg/dm³（II组）和1000mg/dm³（III组）。对照大鼠给予自来水。实验2、4和6周后处死动物。收集胰腺组织进行超微结构分析，并使用原子吸收光谱仪测定血液和胰腺干重中的铅含量。给予醋酸铅6周后，与对照组相比，铅含量增加了5至10倍。超微结构显示，低剂量铅导致染色质边集、核周间隙增宽和腺泡细胞核轮廓折叠。长期给予高剂量铅导致假包涵体和铅-蛋白质复合物核内真实包涵体的形成。这些改变证明了铅对胰腺腺泡细胞核的毒性作用。
