Matsuta M, Kimura S, Kosegawa G, Kon S, Matsuta M
Department of Dermatology, Iwate Medical University, Morioka, Japan.
J Dermatol. 1996 Mar;23(3):147-52. doi: 10.1111/j.1346-8138.1996.tb03989.x.
The expression of the Ki-67 antigen was investigated in 44 epithelial skin tumors using an immunohistochemical technique on formalin-fixed, paraffin-embedded tissue sections. Microwave oven heating was employed for retrieval of the antigen in these tissue sections. The staining patterns varied among the epithelial skin tumors. The assessment of immunohistochemical staining was based upon the growth fraction (GF), defined as the number of Ki-67 positive cells divided by the total number of tumor cells counted and expressed as a percentage. GF was 9.7 +/- 3.1% in seborrheic keratosis, 19.5 +/- 2.9% in keratoacanthoma, 23.1 +/- 4.9% in basal cell carcinoma, 18.5 +/- 6.3% in actinic keratosis, 37.1 +/- 6.0% in Bowen's disease, and 32.9 +/- 10.5% in squamous cell carcinoma. There was a significant difference in GF between the keratoacanthoma and squamous cell carcinoma (p < 0.01). Actinic keratosis showed a relatively low GF, whereas Bowen's disease showed a high one. Furthermore, the GF tended to increase with tumor cell differentiation in squamous cell carcinoma: 23.7% (+/- 5.0) in well-differentiated, 35.0% (+/- 6.2) in moderately-differentiated, and 47.6% (+/- 4.5) in poorly-differentiated squamous cell carcinomas. Immunohistochemistry with MIB-1 may give useful additional information in the differential diagnosis of KA and SCC.
采用免疫组织化学技术，在福尔马林固定、石蜡包埋的组织切片上，对44例上皮性皮肤肿瘤进行Ki-67抗原表达情况的研究。这些组织切片采用微波炉加热来进行抗原修复。上皮性皮肤肿瘤之间的染色模式各不相同。免疫组织化学染色评估基于生长分数（GF），其定义为Ki-67阳性细胞数除以所计数的肿瘤细胞总数，并以百分比表示。脂溢性角化病的GF为9.7±3.1%，角化棘皮瘤为19.5±2.9%，基底细胞癌为23.1±4.9%，光化性角化病为18.5±6.3%，鲍温病为37.1±6.0%，鳞状细胞癌为32.9±10.5%。角化棘皮瘤和鳞状细胞癌的GF存在显著差异（p<0.01）。光化性角化病的GF相对较低，而鲍温病的GF较高。此外，在鳞状细胞癌中，GF倾向于随着肿瘤细胞分化程度增加而升高：高分化鳞状细胞癌为23.7%（±5.0），中分化为35.0%（±6.2），低分化为47.6%（±4.5）。使用MIB-1进行免疫组织化学检查可能会在角化棘皮瘤和鳞状细胞癌的鉴别诊断中提供有用的额外信息。
