Ontogeny of proliferative cells in the rat fundic gland.

The ontogeny of proliferative cells in the rat fundic gland was studied using bromodeoxyuridine (BrdU) immunohistochemistry from day 17.5 of gestation to 8 weeks after birth. This ontogenic process is divided into 4 stages. (1) The late fetal period extending to 0 day of birth: Proliferative cells were scattered throughout all levels of the stratified epithelium in the earliest stage (day 17.5-18.5 of gestation). With the appearance of a primitive gastric pit at day 19.5 of gestation, proliferative cells were more numerous at the base of the fundic gland. Proliferative cells were concentrated in the gland base and were rarely seen in the epithelial surface from day 21.5 of gestation onwards. (2) One day to 2 weeks after birth: As fundic gland growth proceeded, proliferative cells remained concentrated in the gland base. (3) Two to 4 weeks after birth: Proliferative cells left the gland base and moved upward to reach the adult location in the isthmus. (4) Four to 8 weeks after birth: The development of the fundic gland was complete and proliferative cells remained in a narrow proliferative zone in the isthmus.