Yang D H, Tsuyama S, Ge Y B, Wakamatsu D, Ohmori J, Murata F
Department of Anatomy, Faculty of Medicine, Kagoshima University, Japan.
Histol Histopathol. 1997 Jul;12(3):719-27.
The proliferation and migration of stem cells in the developing and adult rat fundic gland have been studied using BrdU immunohistochemistry and BrdU-GSA II (Griffonia-simplicifolia agglutinin-II) double staining. In the developing rat fundic gland, stem cells were first scattered throughout all levels of the epithelia and then concentrated in the depth of the pits. With the elongation and maturation of the fundic glands, stem cells left the gland base and moved upward. By 4 weeks after birth, the development of the fundic gland was completed and stem cells were confined to a narrow proliferative zone in the isthmus, reaching the adult distribution pattern. In the adult rat fundic gland, stem cells in the isthmus differentiated and migrated upward and downward, replacing the surface mucous cells and glandular cells respectively. For upward migration, it took about one week for stem cells to migrate from the isthmus to the surface. For downward migration, it took about two weeks for stem cells to migrate from the isthmus to the neck, and it took 30-36 weeks to reach the gland unit's blind end. Finally stem cells were lost at the deepest level of the glands. The results obtained by simple topographical distribution in the present experiment agreed well with those obtained by quantitative analysis, suggesting the usefulness of BrdU immunohistochemistry for cell kinetic studies.
利用BrdU免疫组织化学和BrdU-GSA II（西非单叶豆凝集素-II）双重染色技术，对发育中和成年大鼠胃底腺中干细胞的增殖和迁移进行了研究。在发育中的大鼠胃底腺中，干细胞最初散布于上皮的各个层面，随后集中于胃小凹的深部。随着胃底腺的伸长和成熟，干细胞离开腺底部并向上移动。出生后4周时，胃底腺发育完成，干细胞局限于峡部的一个狭窄增殖区，达到成年分布模式。在成年大鼠胃底腺中，峡部的干细胞向上和向下分化迁移，分别替代表面黏液细胞和腺细胞。对于向上迁移，干细胞从峡部迁移到表面大约需要一周时间。对于向下迁移，干细胞从峡部迁移到颈部大约需要两周时间，而到达腺单位盲端则需要30 - 36周。最终，干细胞在腺的最深部消失。本实验通过简单地形分布获得的结果与定量分析结果吻合良好，表明BrdU免疫组织化学在细胞动力学研究中具有实用性。
