Henriksson J, Solari A, Rydåker M, Sousa O E, Pettersson U
Department of Medical Genetics, Uppsala University, Sweden.
Parasitology. 1996 Apr;112 ( Pt 4):385-91. doi: 10.1017/s0031182000066610.
The molecular karyotypes of several different protozoan parasites show high intra-species variation, including different kinetoplastids such as Trypanosoma brucei, Trypanosoma cruzi and Leishmania ssp. In this study, the molecular karyotype of Trypanosoma rangeli was examined. To evaluate potential intra-species molecular karyotype variations, 16 different samples were studied by pulsed field gel electrophoresis (PFGE) followed by ethidium bromide staining and hybridizations with 6 different probes. The result showed that different T. rangeli populations are highly polymorphic regarding the molecular karyotype, and thus suggests that PFGE analysis can be used for classification of different T. rangeli isolates. In addition, the molecular karyotype of T. rangeli was compared to molecular karyotypes of other kinetoplastids, and was shown to be distinctly different from that of T. cruzi, but shows some similarities with the karyotype described for T. brucei. Among the probes used one was identified as highly polymorphic, and thus informative for studies of different T. rangeli populations, and another was useful for differentiation between T. rangeli and T. cruzi.
几种不同原生动物寄生虫的分子核型显示出高度的种内变异，包括不同的动基体目寄生虫，如布氏锥虫、克氏锥虫和利什曼原虫属。在本研究中，对兰氏锥虫的分子核型进行了检测。为了评估潜在的种内分子核型变异，通过脉冲场凝胶电泳（PFGE），随后进行溴化乙锭染色以及与6种不同探针杂交，对16个不同样本进行了研究。结果表明，不同的兰氏锥虫群体在分子核型方面具有高度多态性，因此表明PFGE分析可用于不同兰氏锥虫分离株的分类。此外，将兰氏锥虫的分子核型与其他动基体目寄生虫的分子核型进行了比较，结果显示其与克氏锥虫的分子核型明显不同，但与布氏锥虫所描述的核型有一些相似之处。在所使用的探针中，有一个被鉴定为高度多态性，因此对研究不同的兰氏锥虫群体具有信息价值，另一个则有助于区分兰氏锥虫和克氏锥虫。
