一种可在皮克水平检测赭曲霉毒素A的新型单克隆抗体。
A new monoclonal antibody detecting ochratoxin A at the picogram level.
作者信息
Gyöngyösi-Horváth A, Barna-Vetró I, Solti L
机构信息
Agricultural Biotechnology Center, Gödöllö, Hungary.
出版信息
Lett Appl Microbiol. 1996 Feb;22(2):103-5. doi: 10.1111/j.1472-765x.1996.tb01119.x.
Abstract
A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A-BSA. This antibody was of the IgG1 heavy chain subclass with a kappa type light chain. The 50% inhibition value was 0.45 ng ml-1 in a direct competitive ELISA and the detection limit was 42 pg ml-1. This antibody is very specific, cross-reacting only with ochratoxin B (9.3%).
摘要
用赭曲霉毒素A-牛血清白蛋白免疫Balb/c小鼠后产生了一种抗赭曲霉毒素A的单克隆抗体。该抗体为重链IgG1亚类且轻链为κ型。在直接竞争酶联免疫吸附测定中，50%抑制值为0.45纳克/毫升，检测限为42皮克/毫升。该抗体具有高度特异性，仅与赭曲霉毒素B有交叉反应（交叉反应率为9.3%）。
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