Téren J, Varga J, Hamari Z, Rinyu E, Kevei F
Animal Health and Food Control Station, Szeged, Hungary.
Mycopathologia. 1996;134(3):171-6. doi: 10.1007/BF00436726.
One hundred and fifty-seven strains belonging to Aspergillus section Nigri were tested for ochratoxin A production using three different methods: a relatively new immunochemical method based on an enzyme-linked immunosorbent assay (ELISA), thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The monoclonal antibody-based ELISA technique was successfully used to screen for low levels of ochratoxin A in the black Aspergilli without concentrating the culture filtrates. The results were confirmed by TLC and HPLC analysis and chemical derivatization. These latter methods required concentrated filtrates. Ochratoxin A was detected in the culture filtrates of five of the 12 A. carbonarius strains, none of the 45 A. japonicus strains and three of the 100 isolates in the A. niger aggregate (A. foetidus, A. awamori and A. niger.
使用三种不同方法对属于黑曲霉组的157个菌株进行了赭曲霉毒素A产生情况的检测：一种基于酶联免疫吸附测定（ELISA）的相对较新的免疫化学方法、薄层色谱法（TLC）和高效液相色谱法（HPLC）。基于单克隆抗体的ELISA技术成功用于筛选黑曲霉中低水平的赭曲霉毒素A，而无需对培养滤液进行浓缩。结果通过TLC、HPLC分析和化学衍生化得到证实。后两种方法需要浓缩滤液。在12株碳黑曲霉中的5株、45株日本曲霉中的0株以及黑曲霉聚集群（臭曲霉、泡盛曲霉和黑曲霉）的100个分离株中的3株的培养滤液中检测到了赭曲霉毒素A。
