Varga J, Kevei E, Rinyu E, Téren J, Kozakiewicz Z
Department of Microbiology, Attila József University, Szeged, Hungary.
Appl Environ Microbiol. 1996 Dec;62(12):4461-4. doi: 10.1128/aem.62.12.4461-4464.1996.
Ochratoxin production was tested in 172 strains representing species in sections Fumigati, Circumdati, Candidi, and Wentii of the genus Aspergillus by an immunochemical method using a monoclonal antibody preparation against ochratoxin A. Ochratoxin A was detected in Aspergillus ochraceus, A. alliaceus, A. sclerotiorum, A. sulphureus, A. albertensis, A. auricomus, and A. wentii strains. This is the first report of production of ochratoxins in the latter three species. Ochratoxin production by these species was confirmed by high-performance thin-layer chromatography and by high-performance liquid chromatography. The chemical methods also indicated the production of ochratoxin B by all of the Aspergillus strains mentioned above.
采用针对赭曲霉毒素A的单克隆抗体制剂的免疫化学方法，对代表曲霉属烟曲霉组、环梗曲霉组、白曲霉组和文氏曲霉组的172个菌株进行了赭曲霉毒素产生情况的检测。在赭曲霉、蒜曲霉、菌核曲霉、硫黄曲霉、艾氏曲霉、金耳曲霉和文氏曲霉的菌株中检测到了赭曲霉毒素A。这是后三个物种产生赭曲霉毒素的首次报道。通过高效薄层色谱法和高效液相色谱法证实了这些物种产生赭曲霉毒素。化学方法还表明上述所有曲霉菌株均产生赭曲霉毒素B。
