Parekh M, Drake H L, Daniel S L
Department of Biology, University of Mississippi, University, USA.
Lett Appl Microbiol. 1996 Feb;22(2):115-20. doi: 10.1111/j.1472-765x.1996.tb01122.x.
Desulfovibrio desulfuricans ATCC 27774 was screened for reactivity against aromatic compounds during lactate-dependent, nitrate-dissimilating growth. Only aromatic aldehydes (benzaldehyde, 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, vanillin, iso-vanillin and o-vanillin) were reactive and, with the exception of 2-hydroxybenzaldehyde, were stimulatory to lactate-dependent growth. Aromatic aldehydes were transformed to their corresponding benzoate and benzyl alcohol derivatives, with the ratio of benzoate-to-benzyl alcohol derivatives being dependent upon lactate availability. In presence of lactate, aromatic aldehydes were primarily reduced to their corresponding benzyl alcohol derivatives; in the absence of lactate, aromatic aldehydes were mainly oxidized to their corresponding benzoate derivatives. In the absence of nitrate, 3-hydroxybenzaldehyde was neither reduced nor oxidized. These results indicate that D. desulfuricans is competent in the bidirectional transformation of aromatic aldehydes under nitrate-dissimilating conditions and that the direction of transformation (i.e. reduction or oxidation) is regulated by reductant availability.
在依赖乳酸、异化硝酸盐生长过程中，筛选脱硫脱硫弧菌（Desulfovibrio desulfuricans）ATCC 27774对芳香族化合物的反应活性。只有芳香醛（苯甲醛、2-羟基苯甲醛、3-羟基苯甲醛、4-羟基苯甲醛、香草醛、异香草醛和邻香草醛）具有反应活性，且除2-羟基苯甲醛外，均对依赖乳酸的生长有促进作用。芳香醛被转化为相应的苯甲酸盐和苄醇衍生物，苯甲酸盐与苄醇衍生物的比例取决于乳酸的可利用性。在有乳酸存在时，芳香醛主要被还原为相应的苄醇衍生物；在没有乳酸时，芳香醛主要被氧化为相应的苯甲酸盐衍生物。在没有硝酸盐的情况下，3-羟基苯甲醛既不被还原也不被氧化。这些结果表明，脱硫脱硫弧菌在异化硝酸盐条件下能够双向转化芳香醛，且转化方向（即还原或氧化）受还原剂可利用性的调节。
