一株反硝化假单胞菌中甲苯厌氧氧化生成苯甲醇和苯甲醛
Anaerobic toluene oxidation to benzyl alcohol and benzaldehyde in a denitrifying Pseudomonas strain.
作者信息
Altenschmidt U, Fuchs G
机构信息
Angewandte Mikrobiologie, University of Ulm, Germany.
出版信息
J Bacteriol. 1992 Jul;174(14):4860-2. doi: 10.1128/jb.174.14.4860-4862.1992.
Abstract
The denitrifying Pseudomonas strain K172 was grown with a generation time of 6 h to a cell density of 0.4 g (dry weight) per liter with toluene and nitrate as substrates. We found that anaerobic cell suspensions oxidize [14C]toluene first to [14C]benzyl alcohol and subsequently to [14C]benzaldehyde. This proves that the methyl group of toluene is oxidized without molecular oxygen to a hydroxymethyl group.
摘要
反硝化假单胞菌菌株K172以甲苯和硝酸盐为底物，代时为6小时，生长至细胞密度达到每升0.4克（干重）。我们发现厌氧细胞悬浮液首先将[14C]甲苯氧化为[14C]苄醇，随后氧化为[14C]苯甲醛。这证明甲苯的甲基在没有分子氧的情况下被氧化为羟甲基。
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