Thakur I S
Department of Environmental Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, India.
Lett Appl Microbiol. 1996 Feb;22(2):141-4. doi: 10.1111/j.1472-765x.1996.tb01128.x.
A monoclonal antibody prepared against surface antigen of Sphingomonas sp. strain RW1 was used for the direct detection of RW1-like organisms in environmental samples by epifluorescence microscopy and subsequent confirmation by Western blot. Of the 76 samples collected from various sources and probed using epifluorescence, only one sample, effluent from paper and pulp processing, gave a positive result. The effluent was cultured and yielded an organism which, by Western blot analysis, was shown to contain the 28 kDa protein recognized by the monoclonal antibody.
一种针对鞘氨醇单胞菌属菌株RW1表面抗原制备的单克隆抗体，被用于通过落射荧光显微镜直接检测环境样品中的类RW1微生物，随后通过蛋白质免疫印迹法进行确认。从不同来源收集的76个样品，经落射荧光检测，只有一个样品（纸浆和造纸加工废水）呈阳性结果。该废水经培养后得到一种微生物，通过蛋白质免疫印迹分析表明，该微生物含有能被单克隆抗体识别的28 kDa蛋白质。
