Conrad R, Ellington A D
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Anal Biochem. 1996 Nov 15;242(2):261-5. doi: 10.1006/abio.1996.0462.
Nucleic acids that can bind tightly and specifically to protein targets can be selected from random sequence pools. Just as antibodies have been used to detect proteins in a variety of formats, it is possible the nucleic acid binding species (aptamers) could be used to specifically detect proteins. Using a simple filter binding assay, aptamers have been used to detect protein kinase C (PKC) introduced into a cell extract. Aptamers that were relatively nonspecific could be used to simultaneously detect different PKC isozymes, while aptamers with higher specificities could discriminate between related isozymes. These results demonstrate the feasibility of using aptamers as diagnostic tools to detect proteins.
能够与蛋白质靶标紧密且特异性结合的核酸可以从随机序列库中筛选出来。正如抗体已被用于以多种形式检测蛋白质一样，核酸结合分子（适体）有可能被用于特异性检测蛋白质。通过一种简单的滤膜结合测定法，适体已被用于检测引入细胞提取物中的蛋白激酶C（PKC）。相对非特异性的适体可用于同时检测不同的PKC同工酶，而具有更高特异性的适体能够区分相关的同工酶。这些结果证明了使用适体作为检测蛋白质的诊断工具的可行性。
