异前列腺素8-表前列腺素F2α引起的冠状动脉收缩。
Coronary artery constriction by the isoprostane 8-epi prostaglandin F2 alpha.
作者信息
Kromer B M, Tippins J R
机构信息
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London.
出版信息
Br J Pharmacol. 1996 Nov;119(6):1276-80. doi: 10.1111/j.1476-5381.1996.tb16033.x.
Abstract
- This study was undertaken to compare the effects of 8-epi prostaglandin F2 alpha (8-epi PGF2 alpha) to those of prostaglandin F2 alpha (PGF2 alpha) and U46619, a thromboxane mimetic, on ovine, bovine and porcine coronary arteries. 2. 8-epi PGF2 alpha constricted porcine and bovine coronary arteries in a concentration-dependent manner with EC50 values of 689.0 +/- 229.3 and 1361.0 +/- 272.3 nM, respectively, but had no effect on ovine coronary arteries. 3. U46619 was a potent vasoconstrictor of porcine, ovine and bovine coronary arteries with EC50 values of 33.0 +/- 23.5, 373.3 +/- 69.7 and 254.1 +/- 134.3 nM, respectively. Emax values were significantly greater than those obtained with 8-epi PGF2 alpha. 4. PGF2 alpha constricted procine and bovine coronary arteries in a concentration-dependent manner with EC50 values of 1631.0 +/- 207.6 and 3644.0 +/- 344.8 nM, respectively, but had no effect on ovine coronary arteries. 5. Concentration-dependent constriction to U46619 in porcine coronary arteries was competitively inhibited by SQ29548 (10(-8) M to 10(-7) M) and BM13505 (10(-8) M to 10(-6) M) with no decrease in maximal responses. 6. Concentration-dependent constriction to 8-epi PGF2 alpha in porcine coronary arteries was inhibited in a concentration-dependent manner by SQ29548 (10(-8) M to 10(-7) M) and BM13505 (10(-8) M to 10(-6) M). However, the inhibition was associated with a decrease in maximal response. 7. Maximal responses of porcine coronary artery to U46619 (1 microM) and 8-epi PGF2 alpha (30 microM) were inhibited in a concentration-dependent manner by SQ29548 with IC50 values 99 +/- 12.36 nM and 46.5 +/- 18.67 nM, respectively. 8. Although ovine coronary arteries did not constrict to 8-epi PGF2 alpha pre-incubation of these vessels with 8-epi PGF2 alpha caused a rightward shift of the U46619 response curve in a concentration-dependent manner. 9. Pre-incubation of porcine coronary arteries with 8-epi PGF2 alpha competitively inhibited responses to U46619 with a Schild slope of 0.99 and a pA2 of 6.13. 10. We conclude that 8-epi PGF2 alpha is a vasoconstrictor within porcine and bovine coronary arteries, with a potency approximately twice that of PGF2 alpha but 5-20 times lower than U46619. The data suggest that 8-epi PGF2 alpha is acting as a partial agonist on the TP-receptor in the coronary vasculature.
摘要
- 本研究旨在比较8-表前列腺素F2α（8-epi PGF2α）与前列腺素F2α（PGF2α）以及血栓素类似物U46619对绵羊、牛和猪冠状动脉的作用。2. 8-epi PGF2α以浓度依赖性方式收缩猪和牛的冠状动脉，其半数有效浓度（EC50）值分别为689.0±229.3 nM和1361.0±272.3 nM，但对绵羊冠状动脉无作用。3. U46619是猪、绵羊和牛冠状动脉的强效血管收缩剂，其EC50值分别为33.0±23.5 nM、373.3±69.7 nM和254.1±134.3 nM。最大效应值（Emax）显著大于8-epi PGF2α所获得的值。4. PGF2α以浓度依赖性方式收缩猪和牛的冠状动脉，其EC50值分别为1631.0±207.6 nM和3644.0±344.8 nM，但对绵羊冠状动脉无作用。5. 猪冠状动脉对U46619的浓度依赖性收缩受到SQ29548（10⁻⁸ M至10⁻⁷ M）和BM13505（10⁻⁸ M至10⁻⁶ M）的竞争性抑制，最大反应无降低。6. 猪冠状动脉对8-epi PGF2α的浓度依赖性收缩受到SQ29548（10⁻⁸ M至10⁻⁷ M）和BM13505（10⁻⁸ M至10⁻⁶ M）的浓度依赖性抑制。然而，这种抑制与最大反应的降低有关。7. 猪冠状动脉对U46619（1 μM）和8-epi PGF2α（30 μM）的最大反应受到SQ29548的浓度依赖性抑制，其半数抑制浓度（IC50）值分别为99±12.36 nM和46.5±18.67 nM。8. 虽然绵羊冠状动脉对8-epi PGF2α不收缩，但用8-epi PGF2α预先孵育这些血管会使U46619反应曲线以浓度依赖性方式向右移动。9. 用8-epi PGF2α预先孵育猪冠状动脉会竞争性抑制对U46619的反应，希尔斜率为0.99，pA2为6.13。10. 我们得出结论，8-epi PGF2α是猪和牛冠状动脉内的血管收缩剂，其效力约为PGF2α的两倍，但比U46619低5至20倍。数据表明，8-epi PGF2α在冠状血管系统中作为TP受体的部分激动剂起作用。
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本文引用的文献
1
Thromboxane A2 receptors.血栓素A2受体
J Lipid Mediat Cell Signal. 1995 Oct;12(2-3):361-78. doi: 10.1016/0929-7855(95)00023-j.
2
The isoprostanes. Current knowledge and directions for future research.异前列腺素。当前的认识与未来研究方向。
Biochem Pharmacol. 1996 Jan 12;51(1):1-9. doi: 10.1016/0006-2952(95)02072-1.
3
Marked overproduction of non-cyclooxygenase derived prostanoids (F2-isoprostanes) in the hepatorenal syndrome.
J Lipid Mediat. 1993 Mar-Apr;6(1-3):417-20.
4
Evidence for the existence of F2-isoprostane receptors on rat vascular smooth muscle cells.大鼠血管平滑肌细胞上存在F2-异前列腺素受体的证据。
Am J Physiol. 1993 Jun;264(6 Pt 1):C1619-24. doi: 10.1152/ajpcell.1993.264.6.C1619.
5
Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanes.体内自由基诱导的异前列腺素生成。D环和E环异前列腺素形成的证据。
J Biol Chem. 1994 Feb 11;269(6):4317-26.
6
Evidence that the F2-isoprostane, 8-epi-prostaglandin F2 alpha, is formed in vivo.有证据表明F2-异前列腺素，即8-表前列腺素F2α，是在体内形成的。
Biochim Biophys Acta. 1994 Jan 3;1210(2):244-8. doi: 10.1016/0005-2760(94)90128-7.
7
The free radical system in ischemic heart disease.
Int J Cardiol. 1994 Feb;43(2):121-5. doi: 10.1016/0167-5273(94)90001-9.
8
Oxidative stress in patients with unstable angina.
Eur Heart J. 1994 Feb;15(2):179-83. doi: 10.1093/oxfordjournals.eurheartj.a060473.
9
Actions of the E2-isoprostane, 8-ISO-PGE2, on the platelet thromboxane/endoperoxide receptor in humans and rats: additional evidence for the existence of a unique isoprostane receptor.E2-异前列腺素8-ISO-PGE2对人和大鼠血小板血栓素/内过氧化物受体的作用：存在独特异前列腺素受体的更多证据
Prostaglandins. 1994 Oct;48(4):247-56. doi: 10.1016/0090-6980(94)90011-6.
10
Isoprostanes. Novel markers of endogenous lipid peroxidation and potential mediators of oxidant injury.异前列腺素。内源性脂质过氧化的新型标志物及氧化损伤的潜在介质。
Ann N Y Acad Sci. 1994 Nov 15;744:237-42. doi: 10.1111/j.1749-6632.1994.tb52741.x.
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