来自乳酸发酵短杆菌的隐秘质粒pBL1会导致大肠杆菌生长抑制和丝状化。

A cryptic plasmid pBL1 from Brevibacterium lactofermentum causes growth inhibition and filamentation in Escherichia coli.

作者信息

Goyal D, Wachi M, Kijima N, Kobayashi M, Yukawa H, Nagai K

机构信息

Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Plasmid. 1996 Jul;36(1):62-6. doi: 10.1006/plas.1996.0033.

DOI:10.1006/plas.1996.0033

PMID:8938054

Abstract

A shuttle vector composed of pBL1, a 4.46-kb cryptic plasmid from coryneform bacterium Brevibacterium lactofermentum, and Escherichia coli vector pHSG298 was found to inhibit growth and cause cell filamentation in E coli. After construction of several deletions of pBL1, a 1.23-kb AccI-HindIII fragment was found responsible. DNA sequence analysis showed that this fragment contained a 726-bp-long open reading frame (ORF3), encoding a protein with 242 amino acid residues. Corresponding to ORF3, a 28-kDa protein was detected in an in vitro protein synthesis system. ORF3 was dispensable for the stable maintenance of pBL1 in coryneform bacteria.

摘要

一种由来自棒状细菌乳酸发酵短杆菌的4.46 kb隐蔽质粒pBL1和大肠杆菌载体pHSG298组成的穿梭载体被发现可抑制大肠杆菌的生长并导致细胞丝状化。在构建了几个pBL1缺失体后，发现一个1.23 kb的AccI-HindIII片段是造成这种现象的原因。DNA序列分析表明，该片段包含一个726 bp长的开放阅读框（ORF3），编码一个含有242个氨基酸残基的蛋白质。与ORF3相对应，在体外蛋白质合成系统中检测到一种28 kDa的蛋白质。ORF3对于pBL1在棒状细菌中的稳定维持是可有可无的。