Knirel Y A, Moll H, Zähringer U
Forschungszentrum Borstel, Zentrum für Medizin und Biowissenschaften, Germany.
Carbohydr Res. 1996 Oct 31;293(2):223-34. doi: 10.1016/0008-6215(96)00194-2.
A core oligosaccharide was obtained after mild acid degradation of Legionella pneumophila serogroup 1 lipopolysaccharide (LPS). On the basis of chemical, GLC-MS, 1H, and 13C NMR spectroscopic data, it was found that the oligosaccharide obtained is a highly O-acetylated heptasaccharide having the following structure: [formula: see text] where Kdo is 3-deoxy-D-manno-octulosonic acid and QuiNAc is 2-acetamido-2,6-dideoxyglucose. In the LPS, the O-specific polysaccharide chain is linked to position 3 of the terminal rhamnosyl group and is cleaved during degradation of the LPS. The degradation also induced partial migration and partial removal of the O-acetyl group from the terminal rhamnosyl group which, together with the occurrence of the reducing Kdo residue in multiple forms, contributes to the heterogeneity of the isolated core oligosaccharide. No such highly O-acetylated core oligosaccharide has been reported so far for LPS of Gram-negative bacteria.
对嗜肺军团菌血清型1脂多糖（LPS）进行温和酸降解后获得了一种核心寡糖。根据化学、气相色谱 - 质谱、¹H和¹³C核磁共振光谱数据，发现所获得的寡糖是一种高度O - 乙酰化的七糖，具有以下结构：[分子式：见原文]，其中Kdo是3 - 脱氧 - D - 甘露 - 辛酮糖酸，QuiNAc是2 - 乙酰氨基 - 2,6 - 二脱氧葡萄糖。在LPS中，O - 特异性多糖链连接到末端鼠李糖基的3位，并且在LPS降解过程中被切割。降解还导致O - 乙酰基从末端鼠李糖基发生部分迁移和部分去除，这与还原型Kdo残基以多种形式出现一起，导致了分离出的核心寡糖的异质性。迄今为止，尚未有关于革兰氏阴性菌LPS的这种高度O - 乙酰化核心寡糖的报道。
