Knirel Y A, Rietschel E T, Marre R, Zähringer U
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Germany.
Eur J Biochem. 1994 Apr 1;221(1):239-45. doi: 10.1111/j.1432-1033.1994.tb18734.x.
The O-polysaccharide chain of Legionella pneumophila Philadelphia strain 1 (serogroup 1) lipopolysaccharide was investigated by means of 1H- and 13C-NMR spectroscopy, and chemical analysis. It was found to consist of an alpha-(2-->4) interlinked homopolymer of a 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-nonulos onic acid possessing most likely the D-glycero-L-galacto configuration, representing the first example of an acidic homopolymer of a higher sugar of this class. The ladder-like banding pattern exhibiting small distances between individual bands in the SDS/PAGE is compatible with a monosaccharide repeating unit.
通过¹H-和¹³C-核磁共振光谱以及化学分析，对嗜肺军团菌费城1型菌株（血清群1）脂多糖的O-多糖链进行了研究。结果发现它由一种5-脒基-7-乙酰氨基-8-O-乙酰基-3,5,7,9-四脱氧壬糖酸的α-(2→4) 互连均聚物组成，最有可能具有D-甘油-L-半乳糖构型，这是此类高级糖的酸性均聚物的首个实例。在SDS/PAGE中，各条带之间距离较小的梯状条带模式与单糖重复单元相符。
