Stegeman J A, de Jong M C, van der Heijden H M, Elbers A R, Kimman T G
Animal Health Service, Boxtel, The Netherlands.
Res Vet Sci. 1996 Nov;61(3):263-7. doi: 10.1016/s0034-5288(96)90075-2.
The performance of tests for the detection of antibodies to Aujeszky's disease virus glycoprotein E (gE) in a target population was evaluated by constructing and analysing receiver operating characteristic (ROC) curves. These curves assess the discriminating ability of a test over the entire range of test signals. The advantages of applying the analysis to a sample of the target population (all commercial pigs in the Netherlands), as compared to using a panel of test sera, are that the estimates of sensitivity and specificity, the comparisons between tests and the choices of the cut-off values are all relevant for the target population. The results of a gE-ELISA in colostrum (test A) and in a single droplet of whole blood (test B) were compared with the results obtained with the same ELISA in serum (gold standard). The area under the ROC curve, which is a quantitative measure of test performance, was significantly (P < 0.01) smaller with test A than test B or the gold standard, indicating that test B performed better than test A. No significant difference was observed between test B and the gold standard.
通过构建和分析受试者工作特征（ROC）曲线，评估了在目标人群中检测伪狂犬病病毒糖蛋白E（gE）抗体的检测试验性能。这些曲线评估了检测在整个检测信号范围内的区分能力。与使用一组检测血清相比，将该分析应用于目标人群（荷兰所有商业猪）的样本的优势在于，敏感性和特异性估计、检测之间的比较以及临界值的选择对于目标人群都是相关的。将初乳中的gE-ELISA结果（检测A）和一滴全血中的结果（检测B）与血清中相同ELISA获得的结果（金标准）进行比较。ROC曲线下面积是检测性能的定量指标，检测A的该面积显著（P<0.01）小于检测B或金标准，表明检测B的性能优于检测A。检测B与金标准之间未观察到显著差异。
