大鼠空肠食糜长期缺失对胰腺分泌中膳食蛋白质反应的影响。

Lack of response to dietary protein in pancreatic secretion by chronic deprivation of jejunal chyme in rats.

作者信息

Hara H, Kasai T

机构信息

Dept. of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.

出版信息

Scand J Gastroenterol. 1996 Nov;31(11):1125-31.

PMID:8938908

Abstract

BACKGROUND

Exocrine pancreatic secretion is regulated by luminal factors, bile-pancreatic juice (BPJ), and food chyme. The effects of 10 days' chronic deprivation of luminal chyme on the stimulation of exocrine pancreatic secretion by dietary protein were examined in rats with or without BPJ in the jejunum.

METHODS

The jejunum was deprived of ingested food by preparing a whole-jejunum blind loop. The BPJ was diverted by a common bile-pancreatic duct catheter and was returned to the upper jejunum (with BPJ) or ileum (without BPJ).

RESULTS AND CONCLUSION

In rats with the ingested food and BPJ in the jejunum (control), secretion rates in terms of volume, protein, amylase, and trypsin increased more than twofold from the rates in the fasting state after a jejunal injection of peptic hydrolysate of casein. In contrast, the secretions were not increased by the protein injection in the rats deprived of jejunal chyme with or without BPJ in the jejunum. Even in the chyme-deprived group the protein and trypsin secretion of the fasting state was significantly higher in BPJ-diverted rats than in rats with BPJ. These results showed that chronic deprivation of the jejunal chyme impaired responses of the exocrine pancreas to dietary protein but not the hypersecretions of the pancreas by BPJ diversion.

摘要

背景

胰腺外分泌受腔内因素、胆汁胰液（BPJ）和食物糜的调节。在空肠有无BPJ的大鼠中，研究了10天慢性腔内食糜剥夺对膳食蛋白质刺激胰腺外分泌的影响。

方法

通过制备全空肠盲袢使空肠无法摄入食物。通过胆总管导管使BPJ改道，并将其返回至空肠上段（有BPJ）或回肠（无BPJ）。

结果与结论

在空肠中有摄入食物和BPJ的大鼠（对照组）中，空肠注射酪蛋白消化水解物后，体积、蛋白质、淀粉酶和胰蛋白酶的分泌率比禁食状态下的分泌率增加了两倍多。相比之下，在空肠食糜被剥夺且空肠有无BPJ的大鼠中，蛋白质注射并未增加分泌。即使在食糜剥夺组中，禁食状态下BPJ改道大鼠的蛋白质和胰蛋白酶分泌也显著高于有BPJ的大鼠。这些结果表明，空肠食糜的慢性剥夺损害了胰腺外分泌对膳食蛋白质的反应，但并未损害BPJ改道引起的胰腺高分泌。